RabMAb

Anti-Calpain S1抗体[EPR3324] (ab92333)

概述

  • 产品名称Anti-Calpain S1抗体[EPR3324]
    参阅全部 Calpain S1 一抗
  • 描述
    兔单克隆抗体[EPR3324] to Calpain S1
  • 经测试应用适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human, Chinese Hamster
  • 免疫原

    Synthetic peptide (Human)

  • 阳性对照
    • WB: fetal brain or fetal spleen tissue lysate; T47D or 293T cell lysate. IHC: Human kidney tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab92333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
ICC
Flow Cyt
  • 应用说明Flow Cyt: 1/50.
    ICC: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. The use of an HRP/AP polymerized antibody will give a stronger signal.

    WB: 1/1000 - 1/5000. Predicted molecular weight: 28 kDa.

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
    • 序列相似性Contains 5 EF-hand domains.
    • 结构域The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.
      EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
    • 细胞定位Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 30K antibody
      • Calcium activated neutral proteinase antibody
      • Calcium activated neutral proteinase small subunit antibody
      • Calcium dependent protease small subunit 1 antibody
      • Calcium dependent protease small subunit antibody
      • Calcium-activated neutral proteinase small subunit antibody
      • Calcium-dependent protease small subunit 1 antibody
      • Calcium-dependent protease small subunit antibody
      • Calpain 4 antibody
      • Calpain 4 small subunit (30K) antibody
      • Calpain 4 small subunit antibody
      • Calpain regulatory subunit antibody
      • Calpain small polypeptide antibody
      • Calpain small subunit 1 antibody
      • Calpain4 antibody
      • CalpainS1 antibody
      • CANP antibody
      • CANP small subunit antibody
      • CANPS antibody
      • CAPN 4 antibody
      • CAPN S1 antibody
      • CAPN4 antibody
      • CAPNS 1 antibody
      • CAPNS antibody
      • Capns1 antibody
      • CDPS antibody
      • CPNS1_HUMAN antibody
      • CSS 1 antibody
      • CSS1 antibody
      see all

    Anti-Calpain S1 antibody [EPR3324] 图像

    • All lanes : Anti-Calpain S1 antibody [EPR3324] (ab92333) at 1/2000 dilution

      Lane 1 : T47D cell lysate
      Lane 2 : fetal brain lysate
      Lane 3 : fetal spleen lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 28 kDa
      Observed band size : 28 kDa
    • Immunohistochemistry staining of Calpain S1 in formalin-fixed, paraffin-embedded Human kidney tissue using 1/100 ab92333.
    • Overlay histogram showing HeLa cells stained with ab92333 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92333, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-Calpain S1 antibody [EPR3324] (ab92333)参考文献

    This product has been referenced in:
    • Guo Y  et al. Remarkable Reduction of MAP2 in the Brains of Scrapie-Infected Rodents and Human Prion Disease Possibly Correlated with the Increase of Calpain. PLoS One 7:e30163 (2012). WB ; Chinese Hamster . Read more (PubMed: 22272295) »

    See 1 Publication for this product

    Product Wall

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this lot of antibody, particularly as the previous vial has worked.

    I would like to reassure you that this antibody i...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"