Normal human fibroblast; A431 and Madin-Darby bovine kidney cells (MDBK) lysates.
常规说明This product was used in Hornberger W et al.,(2012) Novel approaches in label-free kinetic detection of small molecule ligand binding to receptor proteins using SAW (surface acoustic wave) technology. Poster Presented at Miptec Congress 2012
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
Use at an assay dependent dilution.
功能Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
序列相似性Contains 5 EF-hand domains.
结构域The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer. EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.
细胞定位Cytoplasm. Cell membrane. Translocates to the plasma membrane upon calcium binding.