兔多克隆抗体to Calpain 5
Recombinant fragment, corresponding to a region within amino acids 1-351 of Human Calpain 5 (NP_004046)
A431 and H1299 whole cell lysates; HeLa cells; 293T and HeLaS3 cell lines
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 73 kDa.
1/100 - 1/200.
Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.
Vitreoretinopathy, neovascular inflammatory
Belongs to the peptidase C2 family.
Contains 1 C2 domain. Contains 1 calpain catalytic domain.
Information by UniProt
Calpain htra 3 antibody
Calpain htra-3 antibody
Western blot - Calpain 5 antibody (ab97534)
All lanes : Anti-Calpain 5 antibody (ab97534) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 73 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Calpain 5 antibody (ab97534)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97534 at 1/100 dilution (top panel) and co-stained with Hoescht 33342 (bottom panel).
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