Anti-Calpain 2抗体(ab155666)
Key features and details
- Rabbit polyclonal to Calpain 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Calpain 2抗体
参阅全部 Calpain 2 一抗 -
描述
兔多克隆抗体to Calpain 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within internal sequence amino acids 162-383 of Human Calpain 2 (UniProt ID: P17655).
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阳性对照
- Jurkat and NIH 3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
细胞定位
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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数据库链接
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Omim: 114230 Human
- SwissProt: P17655 Human
- SwissProt: O08529 Mouse
- Unigene: 350899 Human
- Unigene: 19306 Mouse
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别名
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
图片
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Anti-Calpain 2 antibody (ab155666) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Anti-Calpain 2 antibody (ab155666) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells staining Calpain 2 with ab155666 at 1/200 dilution (green). Botton image shows costaining with Hoechst 33342 (blue).
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Immunohistochemical analysis of paraffin-embedded H1299 xenograft staining Calpain 2 with ab155666 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab155666 被引用在 2 文献中.
- Marhuenda E et al. Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing. J Exp Clin Cancer Res 40:139 (2021). PubMed: 33894774
- Saleh A et al. A novel 3D nanofibre scaffold conserves the plasticity of glioblastoma stem cell invasion by regulating galectin-3 and integrin-ß1 expression. Sci Rep 9:14612 (2019). PubMed: 31601895