RabMAb

Anti-Calpain 1抗体[EPR3319] (ab108400)

概述

  • 产品名称Anti-Calpain 1抗体[EPR3319]
    参阅全部 Calpain 1 一抗
  • 描述
    兔单克隆抗体[EPR3319] to Calpain 1
  • 特异性ab108400 recognises the large subunit of Calpain
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Calpain.

  • 阳性对照
    • Human fetal kidney, U937, A431, (D) 293T, and T47D cell lysates; Human kidney tissue; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Predicted molecular weight: 82 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P 1/200.

See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.

For unpurified use at 1/500 - 1/1000.

Flow Cyt 1/380.

For unpurified use at 1/100 - 1/500.





ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50.

For unpurified use at 1/250 - 1/500.

靶标

  • 功能Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • 细胞定位Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain 1, (mu/I) large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain, large polypeptide L1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANP1 antibody
    • CANPL 1 antibody
    • CANPL1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation inducing protein 30 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all

Anti-Calpain 1 antibody [EPR3319] 图像

  • Overlay histogram showing Jurkat cells stained with ab108400 (red line) at 1/380 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (green line) was rabbit monoclonal IgG used under the same conditions.

  • ab108400 staining Calpain 1 in the T-47D cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/70). ab150077(1/500) an Alexa Fluor®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  • ab108400 staining Calpain 1 in Human transitional cell carcinoma of bladder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/200). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  • All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

    Predicted band size : 82 kDa
  • Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution + C6 Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 82 kDa
  • Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + T47-D Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), HRP- conjugated at 1/1000 dilution

    Predicted band size : 82 kDa
  • Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/5000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 82 kDa
  • All lanes : Anti-Calpain 1 antibody [EPR3319] (ab108400) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal kidney cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : T47D cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 82 kDa
  • ab108400, unpurified, at 1/500 dilution staining Calpain in Human kidney by Immunohistochemistry, Paraffin-embedded tissue.

  • ab108400, unpurified, at 1/250 dilution staining Calpain in HeLa cells by Immunofluorescence.

Anti-Calpain 1 antibody [EPR3319] (ab108400)参考文献

This product has been referenced in:
  • Tsai JC  et al. The role of calpain-myosin 9-Rab7b pathway in mediating the expression of Toll-like receptor 4 in platelets: a novel mechanism involved in a-granules trafficking. PLoS One 9:e85833 (2014). WB ; Human . Read more (PubMed: 24489676) »
  • Ozkan G  et al. How does colistin-induced nephropathy develop and can it be treated? Antimicrob Agents Chemother 57:3463-9 (2013). Read more (PubMed: 23629704) »

See all 2 Publications for this product

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