兔多克隆抗体to Calpain 1
This antibody detects the large subunit (80 kDa) of human Calpain 1.
It does not detect the smaller subunit of human Calpain 1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.0.5% BSA, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml.
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains.
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
Information by UniProt
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
Western blot - Anti-Calpain 1 antibody (ab2543)
Anti-Calpain 1 antibody (ab2543) at 1 µg/ml + Natural human Calpain 1 protein (ab91019) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
has not yet been referenced specifically in any publications.
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