Anti-Calpain 1抗体[9A4H8D3] (ab3589)

概述

  • 产品名称Anti-Calpain 1抗体[9A4H8D3]
    参阅全部 Calpain 1 一抗
  • 描述
    小鼠单克隆抗体[9A4H8D3] to Calpain 1
  • 经测试应用适用于: ICC, WB, ICC/IF, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Fish, Rainbow Trout
  • 免疫原

    Full length native protein (purified) corresponding to Cow Calpain 1. Purified bovine skeletal muscle 80 kDa mu-calpain subunit.

  • 表位This antibody recognizes an epitope between amino acids 465-520 (domain III) of Human Mu Calpain.

性能

应用

Our Abpromise guarantee covers the use of ab3589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/100.
WB 1/2000.
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/20.

靶标

  • 功能Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • 细胞定位Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain 1, (mu/I) large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain, large polypeptide L1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANP1 antibody
    • CANPL 1 antibody
    • CANPL1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation inducing protein 30 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all

Anti-Calpain 1 antibody [9A4H8D3] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Calpain 1 with ab3589 at 1/20. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Samples were blocked with 3% BSA in PBS for 30 minutes at room temperature. Samples were incubated with the primary antibody overnight at 4°C. A biotin-conjugated secondary antibody was used. Counterstained with hematoxylin. Right - negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Calpain 1 with ab3589 at 1/20. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Samples were blocked with 3% BSA in PBS for 30 minutes at room temperature. Samples were incubated with the primary antibody overnight at 4°C. A biotin-conjugated secondary antibody was used. Counterstained with hematoxylin. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of U251 cells labeling Calpain 1 (green) with ab3589 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labeling Calpain 1 (green) with ab3589 at 1/20. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling Calpain 1 (green) with ab3589 at 1/100. F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue). Cells were fixed with formaldehyde and incubated with the primary antibody overnight at 4°C. A DyLight 488-conjugated secondary antibody was used. 60X magnification. Right - negative control.

  • ab3589 at a dilution of 1 / 2000 staining Caplain 1 in Mouse lung extract by Western blot.

Anti-Calpain 1 antibody [9A4H8D3] (ab3589)参考文献

This product has been referenced in:
  • Rodríguez-Fernández L  et al. Isoform-specific function of calpains in cell adhesion disruption: studies in postlactational mammary gland and breast cancer. Biochem J 473:2893-909 (2016). Read more (PubMed: 27402795) »
  • Liu D  et al. Activation of calpains mediates early lung neutrophilic inflammation in ventilator-induced lung injury. Am J Physiol Lung Cell Mol Physiol 302:L370-9 (2012). WB ; Mouse . Read more (PubMed: 22140070) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. We do have positive controls available for these antibodies. The Jurkat lysate catalog number is ab7899. This should also be an appropriate control for the anti-RIP3 antibody, as we do not have the HEK293 transfected cells ...

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We discussed ab3589 a few moments ago over the telephone, and I subsequently noticed that, yesterday, you had sumitted the above technical enquiry. To recap, I suggest that you look at the reference on the datasheet (J. Anim. Sci, 76: 2415-2434, 1998.)...

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