Anti-Calpain 1抗体[15C10] (ab49652)


  • 产品名称Anti-Calpain 1抗体[15C10]
    参阅全部 Calpain 1 一抗
  • 描述
    小鼠单克隆抗体[15C10] to Calpain 1
  • 特异性ab49652 recognizes the larege 80 kDa subunit of mu calpain. The antibody recognizes both the native and denatured protein.
  • 经测试应用适用于: ICC/IF, WB, IP, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Purified calpain from human placenta.

  • 阳性对照
    • ICC/IF: HeLa cells.



Our Abpromise guarantee covers the use of ab49652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 80 kDa.
IP Use a concentration of 1 - 2 µg/ml.
ELISA Use a concentration of 0.5 - 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 4 EF-hand domains.
  • 细胞定位Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca2 activated neutral protease antibody
    • Calcium activated neutral proteinase antibody
    • Calcium activated neutral proteinase small subunit antibody
    • Calcium dependent protease small subunit 1 antibody
    • Calcium dependent protease small subunit antibody
    • Calcium-activated neutral proteinase 1 antibody
    • Calpain 1 antibody
    • Calpain 1 large subunit antibody
    • Calpain 1, (mu/I) large subunit antibody
    • Calpain mu type antibody
    • Calpain mu-type antibody
    • Calpain regulatory subunit antibody
    • Calpain small subunit 1 antibody
    • Calpain, large polypeptide L1 antibody
    • Calpain-1 catalytic subunit antibody
    • Calpain-1 large subunit antibody
    • CAN1_HUMAN antibody
    • CANP 1 antibody
    • CANP antibody
    • CANP small subunit antibody
    • CANP1 antibody
    • CANPL 1 antibody
    • CANPL1 antibody
    • CAPN 1 antibody
    • CAPN1 antibody
    • Cell proliferation inducing protein 30 antibody
    • Cell proliferation-inducing gene 30 protein antibody
    • Micromolar Calpain antibody
    • Micromolar-calpain antibody
    • Mu Calpain antibody
    • muCANP antibody
    • muCL antibody
    see all

Anti-Calpain 1 antibody [15C10] 图像

  • ICC/IF image of ab49652 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49652, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Calpain 1 antibody [15C10] (ab49652) at 1/1000 dilution

    Lane 1 : Nuclear tissue lysate prepared from rat heart, left ventricle
    Lane 2 : Nuclear tissue lysate prepared from rat heart, left ventricle

    Lysates/proteins at 30 µg per lane.

    HRP-conjugated donkey anti-mouse polyclonal at 1/10000 dilution
    developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 80 kDa

    Exposure time : 10 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab49652 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab49652, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Calpain 1 antibody [15C10] (ab49652)参考文献

ab49652 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - nuclear (Heart - left ventricle)
Loading amount 30 µg
Specification Heart - left ventricle
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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提交于 Sep 05 2011