概述

  • 产品名称Anti-Calnexin抗体[EPR3632]
    参阅全部 Calnexin 一抗
  • 描述
    兔单克隆抗体[EPR3632] to Calnexin
  • 特异性Recognizes ER membrane, mitochondria and cis-Golgi
  • 经测试应用适用于: ICC/IF, WB, IP, IHC-Pmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Calnexin aa 1-100.

  • 阳性对照
    • WB: HeLa, A431, SH-SY5Y and HepG2 cell lysates IHC-P: Human tonsil tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    References regarding specificity

    Horner SM  et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

    Myhill N  et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

    Yoshimura SI  et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642

性能

应用

Our Abpromise guarantee covers the use of ab92573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 24649404
WB 1/20000 - 1/100000. Predicted molecular weight: 90 kDa.
IP 1/50.
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins.
    • 序列相似性Belongs to the calreticulin family.
    • 细胞定位Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Calnexin antibody
      • CALX_HUMAN antibody
      • CANX antibody
      • CNX antibody
      • FLJ26570 antibody
      • Histocompatibility complex class I antigen binding protein p88 antibody
      • IP90 antibody
      • Major histocompatibility complex class I antigen-binding protein p88 antibody
      • p90 antibody
      see all

    Anti-Calnexin antibody [EPR3632] 图像



    • Predicted band size : 90 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Calnexin knockout HAP1 cell lysate (20 µg)
      Lane 3: THP1 cell lysate (20 µg)
      Lane 4: Raw264.7 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab92573 observed at 80 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab92573 was shown to specifically react with Calnexin when Calnexin knockout samples were used. Wild-type and Calnexin knockout samples were subjected to SDS-PAGE. ab92573 and ab8245 (loading control to GAPDH) were diluted at 1/20 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-Calnexin antibody [EPR3632] (ab92573) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : HepG2 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 90 kDa
    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue using ab92573 at a 1/100 dilution.
    • Immunocytochemistry/Immunofluorescence analysis of human lung carcinoma A549 cells labelling Calnexin with ab92573. Cells were fixed with 4% PFA in PBS for 15 minutes washed three times with PBS, and then washed twice with 25 mM glycine–PBS. The cells were treated with cold methanol and washed three times with 0.3% Triton X-100 in PBS for permeabilization. The fixed cells were blocked with 5% BSA in PBS overnight. An Alexa Fluor® 594-conjugated anti-rabbit was used as the secondary antibody.

    Anti-Calnexin antibody [EPR3632] (ab92573)参考文献

    This product has been referenced in:
    • Mitsuda S  et al. Ursolic acid, a natural pentacyclic triterpenoid, inhibits intracellular trafficking of proteins and induces accumulation of intercellular adhesion molecule-1 linked to high-mannose-type glycans in the endoplasmic reticulum. FEBS Open Bio 4:229-39 (2014). ICC/IF ; Human . Read more (PubMed: 24649404) »

    See 1 Publication for this product

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