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Focus cells (human hepatoma cell line)
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Our Abpromise guarantee covers the use of ab31290 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 67 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab31290 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Immunofluorescence staining of calnexin in HUVEC cells with ab31290. The samples were blocked with 1% BSA in PBS and the nuclei are stained with DAPI. FAM5C was also immuostained (red). The fluorescent signals were visualized with a laser scanning confocal microscope.
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