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His-Tag E. coli expressed N-terminal ER luminal domain of canine calnexin
Our Abpromise guarantee covers the use of ab10286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.|
|ICC/IF||Use at an assay dependent concentration.|
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Paraformaldehyde fixed HeLa cells stained with ab10286. For further details please refer to the customer Abreview.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"