概述

  • 产品名称Anti-Calnexin抗体[3H4A7]
    参阅全部 Calnexin 一抗
  • 描述
    小鼠单克隆抗体[3H4A7] to Calnexin
  • 特异性Recognizes ER membrane, mitochondria and cis-Golgi
  • 经测试应用适用于: Flow Cyt, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human, African Green Monkey
    预测可用于: Mouse, Rat, Hamster, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Calnexin aa 475-493 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    EAAEERPWLWVVYILTVAL

  • 阳性对照
    • HeLa cell lysate
  • 常规说明

    References regarding specificity

    Horner SM  et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353

    Myhill N  et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615

    Yoshimura SI  et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642

性能

应用

Our Abpromise guarantee covers the use of ab66332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA 1/10000.
ICC/IF 1/200 - 1/1000.

靶标

Anti-Calnexin antibody [3H4A7] 图像

  • All lanes : Anti-Calnexin antibody [3H4A7] (ab66332) at 1/2000 dilution

    Lane 1 : Cell lysates prepared from human A431 cells
    Lane 2 : Cell lysates prepared from human Hela cells
    Lane 3 : Cell lysates prepared from human MCF-7 cells
    Lane 4 : Cell lysates prepared from human A549 cells

    Lysates/proteins at 100 µg per lane.

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size : 90 kDa
  • ab66332 at 1/1000 dilution staining Calnexin in human Hela cells by immunocytochemistry/ Immunofluorescence. An Alexa Fluor® conjugated Goat polyclonal to mouse IgG1 was used as secondary antibody. Green colour in image show positive staining with ab66332.

  • Overlay histogram showing HeLa cells stained with ab66332 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66332, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Calnexin antibody [3H4A7] (ab66332)参考文献

This product has been referenced in:
  • Cominelli A  et al. A unique C-terminal domain allows retention of matrix metalloproteinase-27 in the endoplasmic reticulum. Traffic 15:401-17 (2014). ICC/IF ; African Green Monkey . Read more (PubMed: 24548619) »
  • Koganti S  et al. 2-Methoxyestradiol binding of GPR30 down-regulates angiotensin AT1 receptor. Eur J Pharmacol 723C:131-140 (2013). Read more (PubMed: 24262995) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Sample African Green Monkey Cell (COS-7)
Specification COS-7
Permeabilization Yes - Saponin 0.05%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 31 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebra finch Cell lysate - whole cell (ZFL cells)
Loading amount 30 µg
Specification ZFL cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Benjamin Bonneau

Verified customer

提交于 May 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"