重组Anti-Calmodulin 1/2/3抗体[EPR5028] (ab124742)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5028] to Calmodulin 1/2/3
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Calmodulin 1/2/3抗体[EPR5028]
参阅全部 Calmodulin 1/2/3 一抗 -
描述
兔单克隆抗体[EPR5028] to Calmodulin 1/2/3 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- HeLa, Ramos, HCT116 and NIH 3T3 cell lysates.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5028 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124742于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
|
|
Flow Cyt (Intra) |
1/100 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
---|
WB
1/50000 - 1/200000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
Flow Cyt (Intra)
1/100 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
-
相关性
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. -
细胞定位
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. -
数据库链接
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Omim: 114182 Human
- Omim: 114183 Human
- SwissProt: 114180 Human
- SwissProt: P0DP23 Human
- SwissProt: P0DP24 Human
see all -
形式
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. -
别名
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
图片
-
Overlay histogram showing SHSY-5Y cells stained with ab124742 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124742, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
-
All lanes : Anti-Calmodulin 1/2/3 antibody [EPR5028] (ab124742) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab124742 被引用在 8 文献中.
- Mo S et al. Flunarizine suppresses Mycobacterium tuberculosis growth via calmodulin-dependent phagosome maturation. J Leukoc Biol 111:1021-1029 (2022). PubMed: 34533236
- Wei J et al. Ca2+-CaM Dependent Inactivation of RyR2 Underlies Ca2+ Alternans in Intact Heart. Circ Res 128:e63-e83 (2021). PubMed: 33375811
- Søndergaard MT et al. Role of cardiac ryanodine receptor calmodulin-binding domains in mediating the action of arrhythmogenic calmodulin N-domain mutation N54I. FEBS J 287:2256-2280 (2020). PubMed: 31763755
- Søndergaard MT et al. Diminished inhibition and facilitated activation of RyR2-mediated Ca2+ release is a common defect of arrhythmogenic calmodulin mutations. FEBS J 286:4554-4578 (2019). PubMed: 31230402
- Yang Y et al. Improved calcium sensor GCaMP-X overcomes the calcium channel perturbations induced by the calmodulin in GCaMP. Nat Commun 9:1504 (2018). ICC/IF . PubMed: 29666364
- Zhou P et al. Mechanism of nitric oxide and acid-sensing ion channel 1a modulation of panic-like behaviour in the dorsal periaqueductal grey of the mouse. Behav Brain Res 353:32-39 (2018). PubMed: 29953907
- Bond R et al. The unique C terminus of the calcineurin isoform CNAß1 confers non-canonical regulation of enzyme activity by Ca2+ and calmodulin. J Biol Chem 292:16709-16721 (2017). PubMed: 28842480
- Simms BA et al. A novel calmodulin site in the Cav1.2 N-terminus regulates calcium-dependent inactivation. Pflugers Arch 466:1793-803 (2014). WB ; Rat . PubMed: 24352630