Anti-Calmodulin 1/2/3 (phospho T79 + S81)抗体(ab61001)

概述

  • 产品名称
    Anti-Calmodulin 1/2/3 (phospho T79 + S81)抗体
    参阅全部 Calmodulin 1/2/3 一抗
  • 描述
    兔多克隆抗体to Calmodulin 1/2/3 (phospho T79 + S81)
  • 宿主
    Rabbit
  • 特异性
    Detects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81.
  • 经测试应用
    适用于: ICC/IF, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human Calmodulin 1/2/3. The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-T(p)-D-S(p)-E-E)
    Database link: P0DP23

  • 阳性对照
    • IHC-P: human brain tissue. WB: extracts from Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab61001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/500.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P 1/50 - 1/100.

靶标

  • 相关性
    Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.
  • 细胞定位
    Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
  • 数据库链接
  • 形式
    There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase.
  • 别名
    • CALM 1 antibody
    • CALM 2 antibody
    • CALM 3 antibody
    • CALM antibody
    • CALM1 antibody
    • CALM2 antibody
    • CALM3 antibody
    • CALML2 antibody
    • calmodulin 1 (phosphorylase kinase, delta) antibody
    • Calmodulin 1 antibody
    • Calmodulin 2 (phosphorylase kinase, delta) antibody
    • Calmodulin 2 antibody
    • Calmodulin 3 (phosphorylase kinase, delta) antibody
    • Calmodulin 3 antibody
    • CAM 2 antibody
    • CAM 3 antibody
    • CAM I antibody
    • CAM1 antibody
    • CAM2 antibody
    • CAM3 antibody
    • CAMB antibody
    • CAMC antibody
    • CAMI antibody
    • CAMII antibody
    • CAMIII antibody
    • CPVT4 antibody
    • DD132 antibody
    • FLJ99410 antibody
    • LP7057 protein antibody
    • PHKD antibody
    • PHKD2 antibody
    • PHKD3 antibody
    • phosphorylase kinase delta antibody
    • phosphorylase kinase, delta subunit antibody
    see all

图片

  • All lanes : Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001) at 1/500 dilution

    Lane 1 : HeLa cells treated with Hu (1mg/ml)
    Lane 2 : Raw264.7 cells treated with TNFa (1mg/ml)

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 17 kDa

  • ab61001, at a 1/50 dilution, staining bi-phosphorylated Calmodulin on human brain tissue sections, in the presence (right) or absence (left) of the immunizing phosphopeptide.
  • Immunofluorescence analysis of HepG2 cells labeling Calmodulin (Phospho-Thr79+Ser81) with ab61001 at 1/100 dilution. The panel on the left is without the blocking peptide, while the panel on the right includes the blocking peptide.

文献

This product has been referenced in:
  • Pachuau J  et al. Protein kinase CK2 contributes to diminished small conductance Ca2+-activated K+ channel activity of hypothalamic pre-sympathetic neurons in hypertension. J Neurochem 130:657-67 (2014). Read more (PubMed: 24806793) »
  • Seo HS  et al. Phytoestrogens induce apoptosis via extrinsic pathway, inhibiting nuclear factor-kappaB signaling in HER2-overexpressing breast cancer cells. Anticancer Res 31:3301-13 (2011). Read more (PubMed: 21965740) »

See all 2 Publications for this product

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