antigen sequence: GELTPEEEAQ YKKAFSAVDT DGNGTINAQE LGAALKATGK NLSEAQLRKL ISEVDGDGDG EISFQEFLTA, corresponding to N terminal amino acids 3-72 of Human CALML5.
- Human skin tissue. CALML5 over-expression lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||1/250 - 1/500.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Binds calcium. May be involved in terminal differentiation of keratinocytes.
Particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation. Very low expression in lung.
Contains 4 EF-hand domains.
Information by UniProt
- CALL5_HUMAN antibody
- CALML5 antibody
- Calmodulin like 5 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CALML5 antibody (ab122665)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane and cytoplasm. Recommended concentration of ab122665 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALML5 antibody (ab122665)
ab122665 at 1/2500 dilution staining CALML5 in paraffin-embedded Human skin tissue by Immunohistochemistry.
Western blot - Anti-CALML5 antibody (ab122665)
All lanes : Anti-CALML5 antibody (ab122665) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : CALML5 over-expression lysate; co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique
has not yet been referenced specifically in any publications.
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