Anti-CALHM2抗体(ab121446)

概述

  • 产品名称Anti-CALHM2抗体
  • 描述
    兔多克隆抗体to CALHM2
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    amino acids 124-185 (RGEAYVCALS EFVDPSSLTA REEHFPSAHA TEILARFPCK ENPDNLSDFR EEVSRRLRYE SQ) of Human CALHM2 (Q9HA72).

  • 阳性对照
    • Human colon tissue; RT-4 and U-251 MG cell lysates; Human Plasma, Liver and Tonsil lysates

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

Anti-CALHM2 antibody 图像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121446 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121446, at 1/400 dilution, staining CALHM2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
  • All lanes : Anti-CALHM2 antibody (ab121446) at 1/250 dilution

    Lane 1 : RT-4 cell lysate.
    Lane 2 : U-251 MG cell lysate.
    Lane 3 : Human Plasma
    Lane 4 : Human liver lysate.
    Lane 5 : Human tonsil lysate.

    Developed using the ECL technique

Anti-CALHM2 antibody (ab121446)参考文献

ab121446 has not yet been referenced specifically in any publications.

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We only have full size vials in stock, so we don't have a small aliquot to send out for testing. However, for cases like this when an antibody has not been test...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"