RabMAb

Anti-Caldesmon抗体[EP789Y] (ab45691)

概述

  • 产品名称Anti-Caldesmon抗体[EP789Y]
    参阅全部 Caldesmon 一抗
  • 描述
    兔单克隆抗体[EP789Y] to Caldesmon
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-term of human Caldesmon.

  • 阳性对照
    • HeLa cell lysate, and human normal prostate tissue.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab45691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 93 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
    • 组织特异性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
    • 序列相似性Belongs to the caldesmon family.
    • 结构域The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
    • 翻译后修饰In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
    • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
    • Information by UniProt
    • 数据库链接
    • 别名
      • CAD antibody
      • CALD 1 antibody
      • CALD1 antibody
      • CALD1_HUMAN antibody
      • Caldesmon 1 antibody
      • Caldesmon 1 Isoform 1 antibody
      • Caldesmon 1 Isoform 2 antibody
      • Caldesmon 1 Isoform 3 antibody
      • Caldesmon 1 Isoform 4 antibody
      • Caldesmon 1 Isoform 5 antibody
      • Caldesmon antibody
      • Caldesmon1 antibody
      • CDM antibody
      • H CAD antibody
      • HCAD antibody
      • L CAD antibody
      • LCAD antibody
      • MGC21352 antibody
      • NAG22 antibody
      see all

    Anti-Caldesmon antibody [EP789Y] 图像

    • Ab45691 (1:250) staining human Caldesmon in human normal prostate tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab45691 (1:250).
    • Anti-Caldesmon antibody [EP789Y] (ab45691) at 1/10000 dilution + HeLa cell lysate (10ug/lane)

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    Anti-Caldesmon antibody [EP789Y] (ab45691)参考文献

    This product has been referenced in:
    • Pieretti AC  et al. A novel in vitro model to study alveologenesis. Am J Respir Cell Mol Biol 50:459-69 (2014). IHC-P . Read more (PubMed: 24066869) »
    • Sun Q  et al. Role of myocyte enhancing factor 2B in epithelial myofibroblast transition of human gingival keratinocytes. Exp Biol Med (Maywood) 237:178-85 (2012). Read more (PubMed: 22302709) »

    See all 2 Publications for this product

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