概述

  • 产品名称Anti-Caldesmon抗体[E89]
    参阅全部 Caldesmon 一抗
  • 描述
    兔单克隆抗体[E89] to Caldesmon
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Caldesmon.

  • 阳性对照
    • NIH3T3,C6 and HeLa cell lysate and human uterus
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Caldesmon antibody (Alexa Fluor® 488) [E89] (ab208116)

    Anti-Caldesmon antibody (Alexa Fluor® 647) [E89] (ab208117)

性能

应用

Our Abpromise guarantee covers the use of ab32330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 93 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/80.

靶标

  • 功能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
  • 组织特异性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • 序列相似性Belongs to the caldesmon family.
  • 结构域The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
  • 翻译后修饰In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAD antibody
    • CALD 1 antibody
    • CALD1 antibody
    • CALD1_HUMAN antibody
    • Caldesmon 1 antibody
    • Caldesmon 1 Isoform 1 antibody
    • Caldesmon 1 Isoform 2 antibody
    • Caldesmon 1 Isoform 3 antibody
    • Caldesmon 1 Isoform 4 antibody
    • Caldesmon 1 Isoform 5 antibody
    • Caldesmon antibody
    • Caldesmon1 antibody
    • CDM antibody
    • H CAD antibody
    • HCAD antibody
    • L CAD antibody
    • LCAD antibody
    • MGC21352 antibody
    • NAG22 antibody
    see all

Anti-Caldesmon antibody [E89] 图像

  • Anti-Caldesmon antibody [E89] (ab32330) at 1/20000 dilution + NIH3T3 cell lysate

    Predicted band size : 93 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) cells labelling Caldesmon with purified ab32330 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei couterstained with DAPI (blue). 

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control. 

  • ab32300, at a 1/250 dilution, staining human Caldesmon in uterus tissue by Immunohistochemistry, Paraffin embedded tissue
  • ab32330, at a 1/250 dilution, staining human Caldesmon in HeLa cells by Immunofluorescence
  • Overlay histogram showing HeLa cells stained with ab32330 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32330, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • Fluorescent immunohistochemical analysis of paraffin-embedded human normal kidney vessels using ab32330. Green-Caldesmon red-PI

  • Fluorescent immunohistochemical analysis of paraffin-embedded human tissue using ab32330. Green-Caldesmon red-PI.

  • Fluorescent immunohistochemical analysis of paraffin-embedded human normal uterus tissue using ab32330. Green-Caldesmon red-PI.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-Caldesmon antibody [E89] (ab32330)参考文献

This product has been referenced in:
  • Cyr AR  et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24469049) »
  • Nyp MF  et al. TRIP-1 via AKT modulation drives lung fibroblast/myofibroblast trans-differentiation. Respir Res 15:19 (2014). WB ; Human . Read more (PubMed: 24528651) »

See all 13 Publications for this product

Product Wall

I have attached an image of our Flow cytometry results with ab32330.

We used untreated HeLa cells as our negative control and Anisomycin-treated HeLa cells as our positive control. The primary dilution was 1:20. Both were permeabilized accord...

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Thank you for your enquiry regarding ab32330.

Generally, antibodies can be stored at -20°C for several years if the storage buffer contains cryoprotective agent and the aliquot size is no smaller than 10 µl. Please always check the ...

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Thank you for your reply and for kindly providing the further information.

We have one antibody for detection of Caldesmon which has been tested in flow cytometry and mouse, which I think is the one you already found:

Caldesmon an...

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Thank you for contacting us.

Checking the immunogen sequence for this antibody, this antibody is not specific to Caldesmon isoform 1. Based on the sequence, it might crossreact with isoform 1-6 of human Caldesmon protein.


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Thank you for contacting us. Our source for this antibody confirms that the antibody does recognize a 120-150 kDa protein in smooth muscle samples. They do not, have a blot image, however. Please do not hesitate to contact us if you need any more advic...

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Thank you for your patience. The isotype of the anti-Caldesmon antibody is simply rabbit IgG. In immunology, no specific isotype has been determined for rabbits. It is only known for human and mouse. I hope this information is helpful to you....

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