概述

  • 产品名称Anti-Caldesmon抗体
    参阅全部 Caldesmon 一抗
  • 描述
    兔多克隆抗体to Caldesmon
  • 特异性ab58502 detects endogenous levels of total Caldesmon protein.
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Caldesmon around the phosphorylation site of serine 789 (V-T-SP-P-T).

  • 阳性对照
    • HeLa cell extracts treated with EGF (200ng/ml, 30mins).

性能

应用

Our Abpromise guarantee covers the use of ab58502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).

靶标

  • 功能Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping.
  • 组织特异性High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
  • 序列相似性Belongs to the caldesmon family.
  • 结构域The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
  • 翻译后修饰In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAD antibody
    • CALD 1 antibody
    • CALD1 antibody
    • CALD1_HUMAN antibody
    • Caldesmon 1 antibody
    • Caldesmon 1 Isoform 1 antibody
    • Caldesmon 1 Isoform 2 antibody
    • Caldesmon 1 Isoform 3 antibody
    • Caldesmon 1 Isoform 4 antibody
    • Caldesmon 1 Isoform 5 antibody
    • Caldesmon antibody
    • Caldesmon1 antibody
    • CDM antibody
    • H CAD antibody
    • HCAD antibody
    • L CAD antibody
    • LCAD antibody
    • MGC21352 antibody
    • NAG22 antibody
    see all

Anti-Caldesmon antibody 图像

  • All lanes : Anti-Caldesmon antibody (ab58502) at 1/500 dilution

    Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
    Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
  • Ab58502 staining Human normal skin. Staining is localised to the cytoplasm.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Caldesmon antibody (ab58502)参考文献

This product has been referenced in:
  • Sharkey JT  et al. Melatonin sensitizes human myometrial cells to oxytocin in a protein kinase C alpha/extracellular-signal regulated kinase-dependent manner. J Clin Endocrinol Metab 95:2902-8 (2010). WB ; Human . Read more (PubMed: 20382690) »

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