纯化说明ab84862 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter serilised through a 0.22 µm membrane.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 52 kDa.
Use at an assay dependent dilution.
功能May play a role in ruffle formation and actin cytoskeleton organization. Seems to negatively regulate constitutive secretion.
组织特异性Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.
细胞定位Cytoplasm > perinuclear region. Golgi apparatus. Cytoplasm > cytoskeleton. According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.