Anti-Calcium Sensing Receptor (phospho T888)抗体(ab62214)

概述

  • 产品名称Anti-Calcium Sensing Receptor (phospho T888)抗体
    参阅全部 Calcium Sensing Receptor 一抗
  • 描述
    兔多克隆抗体to Calcium Sensing Receptor (phospho T888)
  • 特异性ab62214 antibody detects endogenous levels of Calcium Sensing Receptor only when phosphorylated at threonine 888.
  • 经测试应用适用于: ICC/IF, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human Calcium Sensing Receptor around the phosphorylation site of threonine 888 (R-A-TP-L-R).

  • 阳性对照
    • HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab62214 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • 组织特异性Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • 疾病相关Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • 序列相似性Belongs to the G-protein coupled receptor 3 family.
  • 翻译后修饰N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Anti-Calcium Sensing Receptor (phospho T888) antibody 图像

  • Immunofluorescence analysis of HeLa cells, using ab62214 (1/500 - 1/1000) staining Calcium Sensing Receptor; in the presence and absence of immunizing peptide.
  • Ab62214 staining human normal renal medulla. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Calcium Sensing Receptor (phospho T888) antibody (ab62214)参考文献

This product has been referenced in:
  • Barradas AM  et al. A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells. Biomaterials 33:3205-15 (2012). ICC/IF ; Human . Read more (PubMed: 22285104) »

See 1 Publication for this product

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