Anti-Calcium Sensing Receptor抗体(ab18200)

概述

  • 产品名称Anti-Calcium Sensing Receptor抗体
    参阅全部 Calcium Sensing Receptor 一抗
  • 描述
    兔多克隆抗体to Calcium Sensing Receptor
  • 经测试应用适用于: WB, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Calcium Sensing Receptor.

    (Peptide available as ab18608.)

  • 阳性对照
    • This antibody gave a positive signal in CASR 293T cell transient overexpression cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab18200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 120-130 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
  • 组织特异性Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
  • 疾病相关Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.
  • 序列相似性Belongs to the G-protein coupled receptor 3 family.
  • 翻译后修饰N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ca sensing receptor antibody
    • Ca2+ sensing receptor 1 antibody
    • Ca2+ sensing receptor antibody
    • CAR antibody
    • CaSR antibody
    • CASR_HUMAN antibody
    • EIG8 antibody
    • Extracellular calcium sensing receptor antibody
    • Extracellular calcium sensing receptor [Precursor] antibody
    • Extracellular calcium-sensing receptor antibody
    • FHH antibody
    • FIH antibody
    • GPRC2A antibody
    • HHC antibody
    • HHC1 antibody
    • Hypocalciuric hypercalcemia 1 antibody
    • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
    • MGC138441 antibody
    • NSHPT antibody
    • Parathyroid Ca(2+) sensing receptor 1 antibody
    • Parathyroid Cell calcium sensing receptor antibody
    • Parathyroid Cell calcium-sensing receptor antibody
    • PCAR 1 antibody
    • PCaR1 antibody
    see all

Anti-Calcium Sensing Receptor antibody 图像

  • All lanes : Anti-Calcium Sensing Receptor antibody (ab18200) at 1 µg/ml

    Lane 1 : CASR 293T Cell Transient Overexpression Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (negative control)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,50 kDa,80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
    In this experiment Hek293 was used as a negative control.
  • This image is courtesy of an Abreview submitted by Dr sriharsha kantamneni

    Lane 1 = HEK cell lysate used as negative control. Lane 2 = Post nuclear fraction of rat brain (P21). As secondary goat anti-rabbit HRP conjugated was used. Please see accompanying abreview for additional information

    See Abreview

Anti-Calcium Sensing Receptor antibody (ab18200)参考文献

ab18200 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Human vascular smooth muscle cell)
Gel Running Conditions Non-reduced Denaturing (8%)
Loading amount 10 µg
Specification Human vascular smooth muscle cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 29 2016

Thank you for calling Abcam earlier today.
I am sorry that you were experiencing issues with ab18200 in western blot. As we discussed, I am sending you ab79038 as free of charge replacement. Your new order number is *.
If there is anything els...

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Thank you for contacting us.

The catalogue number of blocking peptide is ab18608.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (brain sections)
Specification brain sections
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Jul 25 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - other (post nuclear extract of rat brain)
Specification post nuclear extract of rat brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Dr. sriharsha kantamneni

Verified customer

提交于 Oct 31 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"