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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Calcium Sensing Receptor.
(Peptide available as ab18608.)
Our Abpromise guarantee covers the use of ab18200 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 120-130 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
In this experiment Hek293 was used as a negative control.
ab18200 staining Calcium Sensing Receptor in rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in PBST) for 24 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
This antibody gave a weak staining using direct fluorescence, using amplification (secondary biotinylated) produced nice staining. Image taken in the hypothalamic paraventricular nucleus, the staining is not located on the cell membrane but rather on the cytoplasm or nucleus of the cells. B is a higher power magnification of A. The pictures were taken using objectives X10 and X40.
This image is courtesy of an Abreview submitted by Dr sriharsha kantamneniLane 1 = HEK cell lysate used as negative control. Lane 2 = Post nuclear fraction of rat brain (P21). As secondary goat anti-rabbit HRP conjugated was used. Please see accompanying abreview for additional information
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