Anti-Calcium Pump PMCA2 ATPase抗体(ab3529)

概述

  • 产品名称Anti-Calcium Pump PMCA2 ATPase抗体
  • 描述
    兔多克隆抗体to Calcium Pump PMCA2 ATPase
  • 特异性Detects PMCA 2a and 2b ATPase.
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, ICC, IP, WB, ELISA, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human, Pig, Non Human Primates
  • 免疫原

    Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 5-19.
    Sequence:

    TNSDFYSKNQRNESS

  • 阳性对照
    • In western blot, this antibody gave a positive signal in human and rat brain tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20489728
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/20 - 1/200.
ICC Use at an assay dependent concentration.
IP Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • 组织特异性Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.
  • 序列相似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AT2B2_HUMAN antibody
    • atp2b2 antibody
    • Plasma membrane calcium ATPase 2 antibody
    • Plasma membrane calcium ATPase antibody
    • Plasma membrane calcium ATPase isoform 2 antibody
    • Plasma membrane calcium pump isoform 2 antibody
    • Plasma membrane calcium-transporting ATPase 2 antibody
    • PMCA2 antibody
    see all

Anti-Calcium Pump PMCA2 ATPase antibody 图像

  • ab3529 staining Calcium Pump PMCA2 ATPase in Mouse purkinje cells in cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS + 3% NGS + 0.5% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Calcium Pump PMCA2 ATPase antibody (ab3529) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Human brain tissue lysate - total protein (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 137 kDa


    Exposure time : 2 minutes
    There are 8 isoforms of Calcium Pump PMCA2 ATPase (Swissprot). Ab3529 is expected to recognise all isoforms. The predicted molecular weight of the different isoforms ranges from 127-kDa to 138-kDa. The detection of more than one isoform by ab3529 in brain tissue lysates may explain the banding pattern observed. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
  • Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)参考文献

This product has been referenced in:
  • Laporta J  et al. Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice. PLoS One 9:e110190 (2014). Read more (PubMed: 25299122) »
  • Sherkhane P & Kapfhammer JP The Plasma Membrane Ca(2+)-ATPase2 (PMCA2) Is Involved in the Regulation of Purkinje Cell Dendritic Growth in Cerebellar Organotypic Slice Cultures. Neural Plast 2013:321685 (2013). IHC (PFA fixed) ; Mouse . Read more (PubMed: 24288624) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step None
Sample Mouse Tissue sections (Purkinje cells in cerebellum)
Specification Purkinje cells in cerebellum
Permeabilization Yes - 0.5% Triton x100
Fixative Paraformaldehyde
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Mr. Pradeep Sherkhane

Verified customer

提交于 Jul 08 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue lysate - whole (Rat cerebellar slices)
Specification Rat cerebellar slices
Fixative Formaldehyde
Antigen retrieval step None
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Aug 17 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (Rat forebrain and cerebellum preparations, eg syna)
Specification Rat forebrain and cerebellum preparations, eg syna
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Aug 17 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"