Anti-Calcium-independent Phospholipase A2/PLA2G6抗体(ab104416)
Key features and details
- Rabbit polyclonal to Calcium-independent Phospholipase A2/PLA2G6
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Calcium-independent Phospholipase A2/PLA2G6抗体
参阅全部 Calcium-independent Phospholipase A2/PLA2G6 一抗 -
描述
兔多克隆抗体to Calcium-independent Phospholipase A2/PLA2G6 -
宿主
Rabbit -
特异性
Reactive against mammalian transfected lysate. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein within Human Calcium-independent Phospholipase A2/PLA2G6. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_001004426.1 -
阳性对照
- Human kidney tissue lysate, Mouse kidney tissue lysate, Transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104416于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. |
靶标
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功能
Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F-actin polymerization at the pseudopods.
Isoform ankyrin-iPLA2-1 and isoform ankyrin-iPLA2-2, which lack the catalytic domain, are probably involved in the negative regulation of iPLA2 activity. -
组织特异性
Four different transcripts were found to be expressed in a distinct tissue distribution. -
疾病相关
Neurodegeneration with brain iron accumulation 2B
Neurodegeneration with brain iron accumulation 2A
Parkinson disease 14 -
序列相似性
Contains 7 ANK repeats.
Contains 1 patatin domain. -
细胞定位
Cytoplasm and Membrane. Recruited to the membrane-enriched pseudopod upon MCP1/CCL2 stimulation in monocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 8398 Human
- Entrez Gene: 53357 Mouse
- Omim: 603604 Human
- SwissProt: O60733 Human
- SwissProt: P97819 Mouse
- Unigene: 170479 Human
- Unigene: 155620 Mouse
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形式
The isoform LH-iPLA2 is located at the membrane. The isoform SH-iPLA2 is cytoplasmic. -
别名
- 85 kDa calcium independent phospholipase A2 antibody
- 85/88 kDa calcium-independent phospholipase A2 antibody
- BB112799 antibody
see all
图片
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Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml + Human kidney tissue lysate at 50 µg
Predicted band size: 90 kDa -
Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml + Mouse kidney tissue lysate at 50 µg
Predicted band size: 90 kDa -
All lanes : Anti-Calcium-independent Phospholipase A2/PLA2G6 antibody (ab104416) at 1 µg/ml
Lane 1 : Calcium-independent Phospholipase A2/PLA2G6 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 90 kDa
Observed band size: 84 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab104416 尚未被引用在任何文献中。