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Calcium Detection Kit (Colorimetric) (ab102505) provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM). A chromogenic complex is formed between calcium ions and 0-cresolphthalein and measured at OD = 575 nm.
This product detects free calcium ions only.
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Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
|Calcium Assay Buffer||NM||1 x 15ml|
|Calcium Standard||Yellow||1 x 100µl|
|Chromogenic Reagent||NM||1 x 25ml|
Our Abpromise guarantee covers the use of ab102505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Ca2+ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1:8.