Calcium Detection试剂盒(Colorimetric) (ab102505)

概述

  • 产品名称Calcium Detection试剂盒(Colorimetric)
    参阅全部 Calcium 试剂盒
  • 测试
    250 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media
  • 检测类型Quantitative
  • 灵敏度
    > 0.1 mM
  • 范围
    0.1 mM - 25 mM
  • 检测时间
    0h 20m
  • 种属反应性
    与反应: Cat, Human, all Mammals
  • 产品概述

    Calcium Detection Kit (Colorimetric) (ab102505) provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.1 – 25 mM). A chromogenic complex is formed between calcium ions and 0-cresolphthalein and measured at OD = 575 nm.

    This product detects free calcium ions only.


    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism.
  • 别名
    • Ca++
    • Ca2+

应用

Our Abpromise guarantee covers the use of ab102505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Calcium Detection Kit (Colorimetric) 图像

  • Ca2+ measured in biologicals showing concentration (micromolar). The samples were testing in a range of diltutios (neat to 1:8).

  • Example of standard curve obtained using ab102505

实验方案

Calcium Detection Kit (Colorimetric) (ab102505)参考文献

This product has been referenced in:
  • Zhao X  et al. Linearly Patterned Programmed Cell Necrosis Induced by Chronic Hypoxia Plays a Role in Melanoma Angiogenesis. J Cancer 7:22-31 (2016). Mouse . Read more (PubMed: 26722356) »
  • Wollein Waldetoft K  et al. Saliva-Induced Clotting Captures Streptococci: Novel Roles for Coagulation and Fibrinolysis in Host Defense and Immune Evasion. Infect Immun 84:2813-23 (2016). Read more (PubMed: 27456827) »

See all 3 Publications for this product

Product Wall


Based on the laboratories experience with the ab102505Calcium Detection Kit, for human serum samples they have either added 10 ul directly or did a 1:2 dilution with assay buffer and added 10 ul to the reaction well.

Please note that this...

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Arabidopsis

Excellent Excellent 5/5 (Ease of Use)
Abreviews
I used the calcium detection buffer to test calcium concentration in Arabidopsis, it works well.
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Abcam user community

Verified customer

提交于 Jul 21 2015

I was not able to obtain the composition of the two buffers (they are considered proprietary) but the laboratory has informed me that the buffer in the calcium assay is unlikely to be compatible with the ALP activity assay.

That said, you migh...

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serum calcium in dogs

Good Good 4/5 (Ease of Use)
Abreviews
Used this product in a standard blank ELISA plate format. Programmed a DiaSorin ETI-MAX to run the procedure. Product worked as specified in dogs with no problems. Have found the curve to be very stable run-to-run.

Overall inter/intra assay precision is 5.9% and 3.6% respectively.
Username

Randy Ringold

Verified customer

提交于 Sep 08 2014

Serum calcium in cats

Good Good 4/5 (Ease of Use)
Abreviews
Used to product to test for serum calciums in cats. Programmed a DiaSorin ETI-MAX to run the procedure on standard ELISA plates. Product performed as specified with an inter/intra assay precision of 4.9% and 3.2% respectively.
Username

Randy Ringold

Verified customer

提交于 Sep 03 2014



Ich habe nun einen Bestätigung von Labor: Der Farbstoff 0-cresolphthalein emittiert zwischen 540-600nm. Die spektralen Eigenschaften von 0-cresolphthalein hängen stark vom pH-Wert und der Bindung an Metallionen wie Calcium ab. Da f&...

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The blood vessel walls (largely Endothelial cells) samples can be homogenized in the assay buffer (contains detergent to help lyse cells) and then spun down to prepare a lysate. Several volumes of this lysate must be tested to make sure the r...

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The sample type of kit as mentioned on datasheet lists tissue extracts, this means the kit is suitable for any kind of tissue sections however you may need to optimize expermental conditions.

I hope this ...

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Thank you for contacting us.

This kit can be used to detect the Calcium with rat serum and cell lysates/supernatant.

To know the price please contact our Indian distributor Allied Scientific.

Allied Scientific Products
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Fresh serum samples are recommended.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"