Anti-Calcium channel L type DHPR alpha 2 subunit抗体(ab62814)

概述

  • 产品名称
    Anti-Calcium channel L type DHPR alpha 2 subunit抗体
    参阅全部 Calcium channel L type DHPR alpha 2 subunit 一抗
  • 描述
    山羊多克隆抗体to Calcium channel L type DHPR alpha 2 subunit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog, Pig不与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide:

    C-QEIFNKYNKDKKVR

    , corresponding to internal sequence amino acids 370-383 of Human Calcium channel L type DHPR alpha 2 subunit

  • 阳性对照
    • Human Brain (Cerebellum) lysate.

性能

应用

Our Abpromise guarantee covers the use of ab62814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    Peptide ELISA: 1/16000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 123 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Plays an important role in excitation-contraction coupling.
    • 组织特异性
      Isoform 1 is expressed in skeletal muscle. Isoform 2 is expressed in the central nervous system. Isoform 2, isoform 4 and isoform 5 are expressed in neuroblastoma cells. Isoform 3, isoform 4 and isoform 5 are expressed in the aorta.
    • 序列相似性
      Belongs to the calcium channel subunit alpha-2/delta family.
      Contains 1 cache domain.
      Contains 1 VWFA domain.
    • 结构域
      The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch.
    • 翻译后修饰
      Proteolytically processed into subunits alpha-2-1 and delta-1 that are disulfide-linked.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CA2D1_HUMAN antibody
      • CACN A2 antibody
      • CACNA2 antibody
      • Cacna2d1 antibody
      • CACNL2A antibody
      • Calcium channel L type alpha 2 polypeptide antibody
      • Calcium channel voltage dependent alpha 2/delta subunit 1 antibody
      • CCHL2A antibody
      • Dihydropyridine receptor alpha 2 subunit antibody
      • Dihydropyridine sensitive L type calcium channel alpha 2/delta subunit antibody
      • Dihydropyridine sensitive L type calcium channel subunits alpha 2/delta antibody
      • L type calcium channel subunit alpha 2 antibody
      • MHS 3 antibody
      • MHS3 antibody
      • Voltage dependent calcium channel subunit alpha 2/delta 1 antibody
      • Voltage gated calcium channel subunit alpha 2/delta 1 antibody
      • Voltage-dependent calcium channel subunit delta-1 antibody
      • Voltage-gated calcium channel subunit alpha-2/delta-1 antibody
      see all

    图片

    • Anti-Calcium channel L type DHPR alpha 2 subunit antibody (ab62814) at 0.03 µg/ml + Human Brain (Cerebellum) lysate (in RIPA buffer) at 35 µg

      Predicted band size : 123 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    This product has been referenced in:
    • Lugenbiel P  et al. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS One 10:e0116395 (2015). WB . Read more (PubMed: 25775120) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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