Anti-Calcitonin receptor抗体(ab11042)

概述

  • 产品名称Anti-Calcitonin receptor抗体
    参阅全部 Calcitonin receptor 一抗
  • 描述
    兔多克隆抗体to Calcitonin receptor
  • 特异性ab11042 recognises the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. This antibody recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.
  • 经测试应用适用于: IHC-Fr, IHC-FoFr, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    KGLPIYICHQEPRNPPVSNN

    , corresponding to amino acids 476-496 of Rat Calcitonin receptor

  • 阳性对照
    • Kidney, cultered osteoclasts.
  • 常规说明Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10 - 1/50.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/10 - 1/50. We recommend the use of 4% paraformaldehyde fixation, rather than formalin fixation. A sensitive detection system is also recommended. e.g. A catalysed signal amplification system. This product does not require protein digestion pre-treatment This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 56.9 kDa.
ELISA 1/10000.

靶标

Anti-Calcitonin receptor antibody (ab11042)参考文献

This product has been referenced in:
  • Bian Z  et al. Cd47-Sirpa interaction and IL-10 constrain inflammation-induced macrophage phagocytosis of healthy self-cells. Proc Natl Acad Sci U S A 113:E5434-43 (2016). Read more (PubMed: 27578867) »
  • Thorfve A  et al. Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant healing in vivo. Acta Biomater 10:1451-62 (2014). Read more (PubMed: 24342040) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain sections)
Specification Brain sections
Permeabilization Yes - 0.3% Triton-X 100
Fixative Paraformaldehyde
Username

Mr. Vaibhav Konanur

Verified customer

提交于 Oct 28 2014


We have only two antibodies anti-human calcitonin receptor guaranteed to work in WB in our catalogue: ab103422 and ab11042.

Both of these should recognise all three human isoforms since there is a large sequence homology between all isof...

Read More

Vielen Dank für Ihre Email.



Leider haben Sie Recht, was das austesten mit der technovit 9100neu angeht, und auch versenden wir keine Proben, da wir bei über 90.000Produkten gar nicht die logistischen Möglichkeiten habe...

Read More

Thank you for your enquiry. Ab11042 was raised against a synthetic peptide with the following sequence: KGLPIYICHQEPRNPPVSNN-NH2. I have updated the datasheet with this information for future reference. I hope this information helps. Please do...

Read More

I have just received the following information from the source of ab11042: "The cross-reactivity of this antibody with the human calcitonin receptor has been demonstrated by the antibody originator using human foetal kidney (pathology specimens), fi...

Read More

I'm sorry to hear you are having problem with ab11042. I would like to suggest trying the immunohistochemistry steps without antigen retrieval prior to staining of paraffin embedded sections. I would also recommend using triton (0.3%v/v)in PBS in ...

Read More

Thank you for your enquiry. This antibody is specific for the calcitonin receptor. This receptor is expressed by osteoclasts but expression has also been reported in the kidney, central and peripheral nervous systems. We hope that this information ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"