Anti-Calcitonin receptor抗体(ab11042)


  • 产品名称
    Anti-Calcitonin receptor抗体
    参阅全部 Calcitonin receptor 一抗
  • 描述
    兔多克隆抗体to Calcitonin receptor
  • 特异性
    ab11042 recognises the calcitonin receptor in human, mouse and rat. The calcitonin receptor is a seven transmembrane domain cell surface protein which is coupled to G protein messenger systems. This antibody recognises an epitope within the cytoplasmic domain that is common to both C1a and C1b rat isoforms.
  • 经测试应用
    适用于: IHC-Fr, IHC-FoFr, IHC-P, WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 476-496 of Rat Calcitonin receptor

  • 阳性对照
    • Kidney, cultered osteoclasts.
  • 常规说明
    Should this product contain a precipitate we recommend microcentrifugation before use.



Our Abpromise guarantee covers the use of ab11042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/10 - 1/50.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/10 - 1/50. We recommend the use of 4% paraformaldehyde fixation, rather than formalin fixation. A sensitive detection system is also recommended. e.g. A catalysed signal amplification system. This product does not require protein digestion pre-treatment This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 56.9 kDa.
ELISA 1/10000.


Anti-Calcitonin receptor antibody 图像

  • ab11042 staining Calcitonin receptor in Rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X, blocked with 5% serum for 45 minutes at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 18 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody

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Anti-Calcitonin receptor antibody (ab11042)参考文献

This product has been referenced in:
  • Bian Z  et al. Cd47-Sirpa interaction and IL-10 constrain inflammation-induced macrophage phagocytosis of healthy self-cells. Proc Natl Acad Sci U S A 113:E5434-43 (2016). Read more (PubMed: 27578867) »
  • Xing R  et al. Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL. Int J Mol Med 38:1125-34 (2016). Read more (PubMed: 27599586) »

See all 7 Publications for this product

Product Wall

Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
Rat Tissue sections (Brain sections)
Brain sections
Yes - 0.3% Triton-X 100

Mr. Vaibhav Konanur

Verified customer

提交于 Oct 28 2014

We have only two antibodies anti-human calcitonin receptor guaranteed to work in WB in our catalogue: ab103422 and ab11042.

Both of these should recognise all three human isoforms since there is a large sequence homology between all isof...

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Vielen Dank für Ihre Email.

Leider haben Sie Recht, was das austesten mit der technovit 9100neu angeht, und auch versenden wir keine Proben, da wir bei über 90.000Produkten gar nicht die logistischen Möglichkeiten habe...

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Thank you for your enquiry. Ab11042 was raised against a synthetic peptide with the following sequence: KGLPIYICHQEPRNPPVSNN-NH2. I have updated the datasheet with this information for future reference. I hope this information helps. Please do...

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I have just received the following information from the source of ab11042: "The cross-reactivity of this antibody with the human calcitonin receptor has been demonstrated by the antibody originator using human foetal kidney (pathology specimens), fi...

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I'm sorry to hear you are having problem with ab11042. I would like to suggest trying the immunohistochemistry steps without antigen retrieval prior to staining of paraffin embedded sections. I would also recommend using triton (0.3%v/v)in PBS in ...

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Thank you for your enquiry. This antibody is specific for the calcitonin receptor. This receptor is expressed by osteoclasts but expression has also been reported in the kidney, central and peripheral nervous systems. We hope that this information ...

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