概述

  • 产品名称
    Anti-Calcitonin抗体
    参阅全部 Calcitonin 一抗
  • 描述
    兔多克隆抗体to Calcitonin
  • 经测试应用
    适用于: Sandwich ELISA, ELISA, IHC-FoFr, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Horse, Dog, Pig
  • 免疫原

    Synthetic human calcitonin 1-32.

  • 阳性对照
    • Medullary carcinoma of the thyroid or thyroid c-cell hyperplasia.
  • 常规说明
    Prolonged fixation in buffered formalin can destroy the epitope

性能

应用

Our Abpromise guarantee covers the use of ab8553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [16B5] to Calcitonin (ab11493). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab11493 as Capture.
ELISA 1/300 - 1/3000.
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/75 - 1/150. Antigen retrieval is not essential but may optimise staining.
WB 1/200 - 1/500. Predicted molecular weight: 15 kDa.

靶标

  • 相关性
    Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.
  • 细胞定位
    Cytoplasmic and Secreted
  • 数据库链接
  • 别名
    • Alpha CGRP antibody
    • alpha type CGRP antibody
    • CALC 1 antibody
    • CALC A antibody
    • CALC1 antibody
    • calcitonin 1 antibody
    • calcitonin gene related peptide 1 antibody
    • calcitonin gene related peptide I antibody
    • calcitonin related polypeptide alpha antibody
    • CGRP 1 antibody
    • CGRP antibody
    • CGRP I antibody
    • CGRP1 antibody
    • CT antibody
    • katacalcin antibody
    • KC antibody
    see all

Anti-Calcitonin antibody 图像

  • Standard Curve for Calcitonin dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [16B5 ] to Calcitonin (ab11493) at 5ug/ml and Detector Antibody Rabbit polyclonal to Calcitonin (ab8553) at 0.5ug/ml
  • Anti-Calcitonin antibody (ab8553) at 1/25 dilution + Human thyroid lysate.

    Predicted band size : 15 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of human thyroid staining with ab8553.

Anti-Calcitonin antibody (ab8553)参考文献

This product has been referenced in:
  • Yerbury JJ  et al. alpha2-Macroglobulin and haptoglobin suppress amyloid formation by interacting with prefibrillar protein species. J Biol Chem 284:4246-54 (2009). WB ; Human . Read more (PubMed: 19074141) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Thyroid gland)
Antigen retrieval step
None
Permeabilization
No
Specification
Thyroid gland
Blocking step
Goat normal serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 24°C
Fixative
Formaldehyde
Username

Bertram

Verified customer

提交于 Mar 20 2017

Thank you for your patience. The originator of ab8553 has informed me that Calcitonin is a small peptide with 32 amino acids, MW 3417 Daltons. You also previously enquired regarding ab8571, Mouse monoclonal [ B34.1] to Thyroglobulin (FITC). Thyrogl...

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Reactivity against procalcitonin has not been specifically tested, however it may work provided the epitope recognised in calcitonin is not masked in the procalcitonin molecule. The epitope of this antibody has not been mapped, however the antibody was...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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