使用敲除细胞株进行验证RabMAb

Anti-Calcineurin A抗体[EPR1670(2)] (ab109412)

概述

  • 产品名称Anti-Calcineurin A抗体[EPR1670(2)]
    参阅全部 Calcineurin A 一抗
  • 描述
    兔单克隆抗体[EPR1670(2)] to Calcineurin A
  • 经测试应用适用于: WB, Flow Cyt, ICC/IFmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of Human Calcineurin A

  • 阳性对照
    • Fetal brain, SH-SY5Y, A431, and HeLa cell lysates; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-P or IP.
  • 靶标

    • 功能Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
    • 序列相似性Belongs to the PPP phosphatase family. PP-2B subfamily.
    • 细胞定位Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha isoform formerly PPP2B antibody
      • Calcineurin A alpha antibody
      • Calcineurin A1 antibody
      • CalcineurinA antibody
      • Calmodulin dependent calcineurin A subunit alpha isoform antibody
      • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
      • CALN antibody
      • CALNA 1 antibody
      • CALNA antibody
      • CALNA1 antibody
      • CAM PRP catalytic subunit antibody
      • CAM-PRP catalytic subunit antibody
      • CCN 1 antibody
      • CCN1 antibody
      • CNA 1 antibody
      • CNA alpha antibody
      • CNA antibody
      • CNA1 antibody
      • PP2BA_HUMAN antibody
      • PPP2B antibody
      • Ppp3ca antibody
      • Protein phosphatase 2B catalytic subunit antibody
      • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
      • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
      • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
      • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
      • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
      see all

    Anti-Calcineurin A antibody [EPR1670(2)] 图像



    • Predicted band size : 59 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)

      Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)

      Lane 3: A431 cell lysate (20 µg)

      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109412 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab109412 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab109412 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

      Lane 1 : fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : A431 lysate
      Lane 4 : HeLa cells lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 59 kDa
      Observed band size : 58 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using 1/100 ab109412.

    Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)参考文献

    ab109412 has not yet been referenced specifically in any publications.

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