使用敲除细胞株进行验证RabMAb

Anti-Calcineurin A抗体[EP1669Y] (ab52761)

概述

  • 产品名称
    Anti-Calcineurin A抗体[EP1669Y]
    参阅全部 Calcineurin A 一抗
  • 描述
    兔单克隆抗体[EP1669Y] to Calcineurin A
  • 特异性
    This antibody reacts with Calcineurin A.
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human
  • 免疫原

    corresponding to Human Calcineurin A (N terminal).

  • 阳性对照
    • WB: Hela cell lysate.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab52761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP 1/100.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能
      Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
    • 序列相似性
      Belongs to the PPP phosphatase family. PP-2B subfamily.
    • 细胞定位
      Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha isoform formerly PPP2B antibody
      • Calcineurin A alpha antibody
      • Calcineurin A1 antibody
      • CalcineurinA antibody
      • Calmodulin dependent calcineurin A subunit alpha isoform antibody
      • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
      • CALN antibody
      • CALNA 1 antibody
      • CALNA antibody
      • CALNA1 antibody
      • CAM PRP catalytic subunit antibody
      • CAM-PRP catalytic subunit antibody
      • CCN 1 antibody
      • CCN1 antibody
      • CNA 1 antibody
      • CNA alpha antibody
      • CNA antibody
      • CNA1 antibody
      • PP2BA_HUMAN antibody
      • PPP2B antibody
      • Ppp3ca antibody
      • Protein phosphatase 2B catalytic subunit antibody
      • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
      • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
      • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
      • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
      • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
      see all

    图片



    • Predicted band size : 59 kDa
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 59 kDa
      Observed band size : 59 kDa
    • Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献

    ab52761 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Tissue lysate - whole (CNS tissue)
    Specification
    CNS tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 01 2014

    Application
    Western blot
    Loading amount
    75 µg
    Gel Running Conditions
    Reduced Denaturing (15)
    Sample
    Mouse Tissue lysate - whole (brain Tissue)
    Specification
    brain Tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 13 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Hamster Cell lysate - whole cell (CHO)
    Loading amount
    5 µg
    Specification
    CHO
    Gel Running Conditions
    Reduced Denaturing (10-20% tris-glycine gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    提交于 Aug 13 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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