Anti-Calcineurin A抗体(ab71149)

Abreviews (1)Q&A (2)Specific References (2)

概述

  • 产品名称
    Anti-Calcineurin A抗体
    参阅全部 Calcineurin A 一抗
  • 描述
    兔多克隆抗体to Calcineurin A
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 521) of human Calcineurin A (NP_000935.1).

  • 阳性对照
    • Whole cell lysate from hela cells, NIH3T3 cells and 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab71149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能
    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • 细胞定位
    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab71149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcineurin A antibody (ab71149)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Calcineurin A with ab71149 at 1/1000 (1µg/ml). Detection: DAB.
  • Western blot - Calcineurin A antibody (ab71149)
    Western blot - Calcineurin A antibody (ab71149)
    All lanes : Anti-Calcineurin A antibody (ab71149) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    Predicted band size: 59 kDa
    Observed band size: 59 kDa

  • Immunoprecipitation - Calcineurin A antibody (ab71149)
    Immunoprecipitation - Calcineurin A antibody (ab71149)
    Immunoprecipitation/ Western Blot of Calcineurin A
    Lane 1: ab71149 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed with 1 µg/ml ab71149.
    Chemiluminescence with an exposure time of 30 seconds.

文献

This product has been referenced in:
  • Haryuna TS  et al. Curcumin Reduces the Noise-Exposed Cochlear Fibroblasts Apoptosis. Int Arch Otorhinolaryngol 20:370-376 (2016). IHC ; Rat . Read more (PubMed: 27746842) »
  • Thoms KM  et al. Cyclosporin A, but not everolimus, inhibits DNA repair mediated by calcineurin: implications for tumorigenesis under immunosuppression. Exp Dermatol 20:232-6 (2011). WB ; Human . Read more (PubMed: 21323745) »

See all 2 Publications for this product

发表研究结果有使用 ab71149？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

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Votes: Highest First

Thanks a lot for your prompt reply. Could you please let me know if this antibody is specific to calcineurin A alpha or calcineurin A beta?

Thank you for your reply. This antibody is specific for the calcineurin A alpha isoform: http://www.uniprot.org/uniprot/Q08209 I hope this information helps. Please contact us with any other questions.

I am using the newly purchased anti-calcineurin A antibody (ab71149), according to the data sheet, I should see a band at 61 kDa (observed size & 59 kDa being the predicted size). But I see two strong bands at around 60 kDa and another band at 5...

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Thank you for your inquiry. We have not seen a banding pattern similar to what you're describing with in-house testing. We have predominantly seen a single band at 60 kDa. However, we have heard from a customer who saw something similar to wha...

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Western blot abreview for Anti-Calcineurin A antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount
50 µg
Specification
GM00637 fibroblast cell line
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
(agent) for 30 minute(s) · Concentration: 5µg/mL · Temperature: 20°C
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Username

Mrs. Annika Schäfer

Verified customer

提交于 Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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