Anti-Calcineurin A抗体(ab71149)

概述

  • 产品名称Anti-Calcineurin A抗体
    参阅全部 Calcineurin A 一抗
  • 描述
    兔多克隆抗体to Calcineurin A
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    A synthetic peptide corresponding to a region between residue 470 and the C-terminus (residue 521) of human Calcineurin A (NP_000935.1).

  • 阳性对照
    • Whole cell lysate from hela cells, NIH3T3 cells and 293T cells.

性能

应用

Our Abpromise guarantee covers the use of ab71149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • 序列相似性Belongs to the PPP phosphatase family. PP-2B subfamily.
  • 细胞定位Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

Anti-Calcineurin A antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Calcineurin A with ab71149 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Calcineurin A antibody (ab71149) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


    Predicted band size : 59 kDa
    Observed band size : 59 kDa
  • Immunoprecipitation/ Western Blot of Calcineurin A
    Lane 1: ab71149 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Subsequent WB detection was performed with 1 µg/ml ab71149.
    Chemiluminescence with an exposure time of 30 seconds.

Anti-Calcineurin A antibody (ab71149)参考文献

This product has been referenced in:
  • Thoms KM  et al. Cyclosporin A, but not everolimus, inhibits DNA repair mediated by calcineurin: implications for tumorigenesis under immunosuppression. Exp Dermatol 20:232-6 (2011). WB ; Human . Read more (PubMed: 21323745) »

See 1 Publication for this product

Product Wall

Thank you for your reply. This antibody is specific for the calcineurin A alpha isoform: http://www.uniprot.org/uniprot/Q08209 I hope this information helps. Please contact us with any other questions.

Thank you for your inquiry. We have not seen a banding pattern similar to what you're describing with in-house testing. We have predominantly seen a single band at 60 kDa. However, we have heard from a customer who saw something similar to wha...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (GM00637 fibroblast cell line)
Loading amount 50 µg
Specification GM00637 fibroblast cell line
Gel Running Conditions Reduced Denaturing (10 %)
Blocking step (agent) for 30 minute(s) · Concentration: 5µg/mL · Temperature: 20°C
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Mrs. Annika Schäfer

Verified customer

提交于 Apr 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"