Anti-Calcineurin A抗体(ab3673)

概述

  • 产品名称
    Anti-Calcineurin A抗体
    参阅全部 Calcineurin A 一抗
  • 描述
    兔多克隆抗体to Calcineurin A
  • 经测试应用
    适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Full length Calcineurin A fusion expressed in E. coli.

性能

应用

Our Abpromise guarantee covers the use of ab3673 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent dilution.
WB 1/2000 - 1/5000. Detects a band of approximately 62 kDa.
ICC/IF 1/500.

靶标

  • 功能
    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.
  • 序列相似性
    Belongs to the PPP phosphatase family. PP-2B subfamily.
  • 细胞定位
    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

图片

  • Western Blot of wild type mouse brain homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).

  • All lanes : Anti-Calcineurin A antibody (ab3673) at 1/200 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Lysate prepared from human HUH-7 cells at 15000 cells

    Secondary
    HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution

    Performed under reducing conditions.

    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution + Recombinant Human Calcineurin A protein (ab101870) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds
  • Immunofluorescence analysis of rat dissociated hippocampal cells, staining Calcineurin A with ab3673.

    Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 10% normal goat serum for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 2% normal goat serum) for 1 hour at 23°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Li Z  et al. Calcineurin/nuclear factor-?B signaling mediates isoflurane-induced hippocampal neuroinflammation and subsequent cognitive impairment in aged rats. Mol Med Rep 15:201-209 (2017). WB ; Rat . Read more (PubMed: 27909728) »
  • Seo HH  et al. The role of nuclear factor of activated T cells during phorbol myristate acetate-induced cardiac differentiation of mesenchymal stem cells. Stem Cell Res Ther 7:90 (2016). IP ; Human . Read more (PubMed: 27405982) »

See all 7 Publications for this product

客户评价及客户问答

Thank you for your interest.
Would you be so kind to provide a summary of the project (sample type, species, application, controls etc) you wish to use this antibody?
I would also advise you to visit the following site where the Terms and Con...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as expected and for the inconvenience this has caused. As r...

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Thank you for contacting us.

I can confirm that the Anti-Calcineurin A antibody (ab3673) is a ployclonal antibody. The affinity of these types of antibodies will change with different rounds of immunisation as well as different hosts. With dif...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Dissociated hippocampal cells)
Specification
Dissociated hippocampal cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.25% Triton 5min RT
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Dr. Matthew Grubb

Verified customer

提交于 Oct 24 2012

DISCOUNT CODE: 100%ABR-EXXXX
Expiration date: 25/01/2013
I am very pleased to hear you would like to accept our offer and test ab3673 in ICC in rat hippocampal cells.
This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF ...

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Thank you for your response.
Could you please confirm if the cells are human or murine origin?
As I understand you wish to test this antibody in immunocytochemistry and not immunohistochemistry. What method will be used to dissociate the hipp...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
20 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (vero)
Total protein in input
1e+006 cells
Specification
vero
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Nov 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (huh-7 cells)
Specification
huh-7 cells
Treatment
viral protein expressed
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2009

Thank you for your enquiry. Further to correspondence with the lab I can tell you that the immunogen used for this antibody was a full length calcineurin A protein. I hope this information helps, please do not hesitate to contact us if you need a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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