RabMAb

Anti-Calbindin抗体[EP3478] (ab108404)

概述

  • 产品名称Anti-Calbindin抗体[EP3478]
    参阅全部 Calbindin 一抗
  • 描述
    兔单克隆抗体[EP3478] to Calbindin
  • 经测试应用适用于: WB, IHC-P, ICC/IF, IHC-Frmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • 阳性对照
    • Fetal brain, fetal cerebellum, fetal hypothalamus and fetal pituitary lysates; Human brain tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab108404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-Fr 1/500.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
    • 序列相似性Belongs to the calbindin family.
      Contains 5 EF-hand domains.
    • 结构域This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium.
    • Information by UniProt
    • 数据库链接
    • 别名
      • avian-type antibody
      • CAB27 antibody
      • CALB 1 antibody
      • CALB antibody
      • CALB1 antibody
      • CALB1_HUMAN antibody
      • Calbindin 1 28kDa antibody
      • Calbindin antibody
      • Calbindin D28 antibody
      • D 28K antibody
      • D-28K antibody
      • D28K antibody
      • OTTHUMP00000166027 antibody
      • OTTHUMP00000225441 antibody
      • RTVL H protein antibody
      • Vitamin D dependent calcium binding protein antibody
      • Vitamin D dependent calcium binding protein avian type antibody
      • Vitamin D-dependent calcium-binding protein antibody
      see all

    Anti-Calbindin antibody [EP3478] 图像

    • All lanes : Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : Fetal cerebellum lysate
      Lane 3 : Fetal hypothalamus lysate
      Lane 4 : Fetal pituitary lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 30 kDa
    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) cells labelling Calbindin with ab108404 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at dilution of 1/200. Nuclei were counterstained with DAPI (blue).

      Confocal image showing nuclear and cytoplasmic staining on Neuro2a cultured with 2% FBS.

    • ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).  The image shows specific staining on Purkinje cells of mouse cerebellum.

      Secondary antibody only was used as negative control and is shown in the inset.

    • ab108404 showing positive staining in Normal kidney tissue.

    • ab108404 showing negative staining in Normal heart tissue.

    • ab108404 showing negative staining in Normal colon tissue.

    • ab108404 showing negative staining in Normal liver tissue.

    Anti-Calbindin antibody [EP3478] (ab108404)参考文献

    ab108404 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab108404.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"