Anti-Calbindin抗体[CB-955] (ab82812)
Key features and details
- Mouse monoclonal [CB-955] to Calbindin
- Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
概述
-
产品名称
Anti-Calbindin抗体[CB-955]
参阅全部 Calbindin 一抗 -
描述
小鼠单克隆抗体[CB-955] to Calbindin -
宿主
Mouse -
经测试应用
适用于: IHC-FoFr, WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Synthetic peptide corresponding to Cow Calbindin.
-
阳性对照
- IHC-P: Rat cerebellum.
-
常规说明
This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3258885 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
纯化说明
Purified from hybridoma cell culture is Protein A column. -
克隆
单克隆 -
克隆编号
CB-955 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab82812于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-FoFr | (6) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use at an assay dependent concentration. PubMed: 20542987
|
|
IHC-P | (1) |
Use a concentration of 10 - 20 µg/ml.
|
说明 |
---|
IHC-FoFr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 20542987 |
IHC-P
Use a concentration of 10 - 20 µg/ml. |
靶标
-
功能
Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. -
序列相似性
Belongs to the calbindin family.
Contains 5 EF-hand domains. -
结构域
This protein has four functional calcium-binding sites; potential sites II and VI have lost affinity for calcium. - Information by UniProt
-
数据库链接
- Entrez Gene: 539930 Cow
- Entrez Gene: 793 Human
- Entrez Gene: 12307 Mouse
- Entrez Gene: 83839 Rat
- Omim: 114050 Human
- SwissProt: P04467 Cow
- SwissProt: P05937 Human
- SwissProt: P12658 Mouse
see all -
别名
- avian-type antibody
- CAB27 antibody
- CALB 1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue labelling Calbindin with ab82812 at 10 µg/mL.
-
Lane 1 : Anti-Calbindin antibody [CB-955] (ab82812) at 4 µg/ml
Lane 2 : No primary control
All lanes : MDBK cell extract
Secondary
All lanes : Goat Anti-Mouse IgG (HRP)
Developed using the ECL technique.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (52)
ab82812 被引用在 52 文献中.
- Wang X et al. SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses. MedComm (2020) 4:e198 (2023). PubMed: 36582303
- D'Souza SP et al. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells. J Comp Neurol 530:1247-1262 (2022). PubMed: 34743323
- Chang KC et al. The chromatin repressors EZH2 and Suv4-20h coregulate cell fate specification during hippocampal development. FEBS Lett 596:294-308 (2022). PubMed: 34890048
- Yang X et al. Social Deficits and Cerebellar Degeneration in Purkinje Cell Scn8a Knockout Mice. Front Mol Neurosci 15:822129 (2022). PubMed: 35557557
- Ferdaus MZ et al. KCC3a, a Strong Candidate Pathway for K+ Loss in Alkalemia. Front Cell Dev Biol 10:931326 (2022). PubMed: 35874803