Anti-Calbindin抗体[CB-955] (ab82812)

概述

  • 产品名称Anti-Calbindin抗体[CB-955]
    参阅全部 Calbindin 一抗
  • 描述
    小鼠单克隆抗体[CB-955] to Calbindin
  • 特异性This antibody is specific to a protein of 28 kDa. Calbindin-D-28K is also known as vitamin D-dependent calcium-binding protein or cholecalcin. Calbindin D-28K has a broad tissue distribution; however it exhibits a cell type-specific expression pattern. This antibody reacts specifically with calbindin-D-28K in brain and kidney tissues from human, bovine, goat, porcine, rabbit, dog, cat, rat, mouse, sheep, and guinea pig sources. It binds weakly to chicken calbindin-D-28K.
  • 经测试应用适用于: ICC/IF, IHC-FoFr, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Rabbit, Goat, Guinea pig, Cow, Cat, Human, Pig
  • 免疫原

    Purified bovine kidney calbindin-D-28K

  • 阳性对照
    • Kidney

性能

应用

Our Abpromise guarantee covers the use of ab82812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 20542987
IHC-FoFr 1/2800.
IHC-P 1/15 - 1/30. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

Anti-Calbindin antibody [CB-955] 图像

  • ab82812 staining rat brain (corpus callosum) sections by IHC-FoFr. The animal was perfused with 4% paraformaldehyde and further post fixed with 4% paraformaldehyde overnight. The tissues were cryoprotected with 30% sucrose and sectioned using a cryostat. Staining with ab82812 at a 1/2800 dilution in PBS-Triton (0.3%) with 0.02% azide was performed for 18h at 25°C. A donkey anti-mouse Alexa488 polyclonal antibody at 1/1000 was used as the secondary antibody.

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  • ab82812 staining Calbindin in Mouse brain tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Sections were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 10% normal donkey serum + 0.3 M Glycine for 1 hour at 25°C; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/2500 in 5% serum + 0.2% Triton X-100 in PBS) for 18 hours at 4°C. An AlexaFluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Counterstained with DAPI.

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Anti-Calbindin antibody [CB-955] (ab82812)参考文献

This product has been referenced in:
  • Walsh P  et al. The mechanism of membrane disruption by cytotoxic amyloid oligomers formed by prion protein(106-126) is dependent on bilayer composition. J Biol Chem 289:10419-30 (2014). IHC (PFA fixed) ; Mouse . Read more (PubMed: 24554723) »
  • Mann ZF  et al. A gradient of Bmp7 specifies the tonotopic axis in the developing inner ear. Nat Commun 5:3839 (2014). Read more (PubMed: 24845721) »

See all 8 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate pH 8.5 for 30 min at 80°C followed by 30 min cooling to room temperature
Permeabilization Yes - 0.2% TritonX-100 added to blocking and antibody dilution buffer
Blocking step 10% normal donkey serum + 0.3 M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Lars Riecken

Verified customer

提交于 Jun 22 2012

That's my pleasure. If you do have any further questions please do not hesitate to contact us again.

I wish you all the best with your publication.

I am sorry for the delay in getting back to you.

I can now confirm that the concentration of the antibody is 750 ug/mL. This is the case for most lots of this antibody. If you would like me to check the particular lot which you have used I wo...

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Thank you for getting back to me and sorry for the delay in my reply.

I am looking into whether the concentration of the anti-Calbindin antibody [CB-955] (ab82812) has been determined. The antibody is provided as that collected from ascites a...

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Thank you for contacting us.

I am glad you have found the anti-Calbindin antibody [CB-955] (ab82812) of use in staining your rat brain tissue.

I, like you, have found several references where Calbindin has been used as a neuronal ma...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain - Cerebral Cortex)
Specification Brain - Cerebral Cortex
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

提交于 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Username

Dr. Karine Thibault

Verified customer

提交于 Mar 12 2012

Thank you for your reply. From what I can find out, ab82812 was tested with a 30 minute primary antibody incubation period at room temperature and there is no notes about whether a permeabilzation step was used or not. We do have multiply other Calbind...

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Thank you for contacting Abcam. We have not tested ab82812 in free floating sections, so I am unable to send you a specific protocol for doing Free Floating section staining. However when using paraffin embedded PFA fixed slides, it is advised ...

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