RabMAb

Anti-Cadherin 9抗体[EPR5113] (ab124803)

概述

  • 产品名称Anti-Cadherin 9抗体[EPR5113]
    参阅全部 Cadherin 9 一抗
  • 描述
    兔单克隆抗体[EPR5113] to Cadherin 9
  • 经测试应用适用于: WB, Flow Cytmore details
    不适用于: ICC,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in the intracellular domain of Human Cadherin 9.

  • 阳性对照
    • 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC,IHC-P or IP.
  • 靶标

    Anti-Cadherin 9 antibody [EPR5113] 图像

    • All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 88 kDa
      Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-Cadherin 9 antibody [EPR5113] (ab124803)参考文献

    ab124803 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (ES Cells and Neural cells)
    Permeabilization Yes - 0.4%-1% Triton
    Specification ES Cells and Neural cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Dr. Maneeshi Prasad

    Verified customer

    提交于 Jul 21 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"