概述

  • 产品名称Anti-CAD抗体[EP710Y]
    参阅全部 CAD 一抗
  • 描述
    兔单克隆抗体[EP710Y] to CAD
  • 特异性ab40800 is specific to the N-terminus of CAD.
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CAD aa 1-100 (N terminal).
    Database link: P27708

  • 阳性对照
    • 293 or HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:
    Anti-CAD antibody (Alexa Fluor® 647) [EP710Y] (ab203699)
    Anti-CAD antibody (HRP) [EP710Y] (ab203701)
    Anti-CAD antibody (Alexa Fluor® 568) [EP710Y] (ab208050)
    Anti-CAD antibody (Phycoerythrin) [EP710Y] (ab208994)

性能

应用

Our Abpromise guarantee covers the use of ab40800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/300.
WB 1/1000. Predicted molecular weight: 243 kDa.
IP 1/30 - 1/50.

靶标

  • 相关性Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
  • 细胞定位Cytoplasmic and Nuclear
  • 数据库链接
  • 别名
    • Aspartate carbamoyltransferase antibody
    • Aspartate transcarbamylase antibody
    • CAD protein antibody
    • CAD trifunctional protein antibody
    • Carbamoyl phosphate synthetase 2 antibody
    • CPSase ATCase DHOase antibody
    • Dihydroorotase antibody
    • Glutamine dependent carbamoyl phosphate synthase antibody
    • Multifunctional protein CAD antibody
    see all

Anti-CAD antibody [EP710Y] 图像

  • Anti-CAD antibody [EP710Y] (ab40800) at 1/2000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 243 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-CAD antibody [EP710Y] (ab40800) at 1/1000 dilution (purified) + HeLa cell lysate at 1/20000 dilution

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 243 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HeLa cells with purified ab40800 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab40800 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • ab40800 (purified) at 1/30 immunoprecipitating CAD in 10 μg HeLa (Lanes 1 and 2, observed at 250 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Anti-CAD antibody [EP710Y] (ab40800) at 1/1000 dilution (unpurified) + HEK293 whole cell lysate (ab7902) at 10 µg

    Predicted band size : 243 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Unpurified ab40800 (1/100), staining human CAD (N-term) in HeLA cells by Immunofluorescence.

Anti-CAD antibody [EP710Y] (ab40800)参考文献

This product has been referenced in:
  • DeVito SR  et al. Cytomegalovirus-mediated activation of pyrimidine biosynthesis drives UDP-sugar synthesis to support viral protein glycosylation. Proc Natl Acad Sci U S A 111:18019-24 (2014). Read more (PubMed: 25472841) »
  • Alfonso-Pérez T  et al. Cytoplasmic interaction of the tumour suppressor protein hSNF5 with dynamin-2 controls endocytosis. Oncogene N/A:N/A (2013). Read more (PubMed: 23851497) »

See all 2 Publications for this product

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