概述

  • 产品名称Anti-CAD抗体
    参阅全部 CAD 一抗
  • 描述
    兔多克隆抗体to CAD
  • 经测试应用适用于: IHC-P, WBmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1650-1700 of Human CAD (NP_004332.2).

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab99312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 相关性Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • Aspartate carbamoyltransferase antibody
      • Aspartate transcarbamylase antibody
      • CAD protein antibody
      • CAD trifunctional protein antibody
      • Carbamoyl phosphate synthetase 2 antibody
      • CPSase ATCase DHOase antibody
      • Dihydroorotase antibody
      • Glutamine dependent carbamoyl phosphate synthase antibody
      • Multifunctional protein CAD antibody
      see all

    Anti-CAD antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAD with ab99312 at 1/200 (1µg/ml). Detection: DAB.
    • All lanes : Anti-CAD antibody (ab99312) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysates at 50 µg
      Lane 2 : HeLa whole cell lysates at 15 µg
      Lane 3 : HeLa whole cell lysates at 5 µg
      Lane 4 : 293T whole cell lysates at 50 µg
      Lane 5 : NIH3T3 whole cell lysates at 50 µg


      Predicted band size : 243 kDa


      Exposure time : 3 seconds

    Anti-CAD antibody (ab99312)参考文献

    ab99312 has not yet been referenced specifically in any publications.

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