Caco 2全细胞裂解液(ab3950)

概述

  • 产品名称Caco 2全细胞裂解液
  • 常规说明Cell line: Caco-2 (Human colorectal adenocarcinoma).
    Growth media: Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 20% FBS.
  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Caco-2 cells possess many of the properties of the small intestine. As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa).

应用

Our Abpromise guarantee covers the use of ab3950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
Caco-2 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isr ecommended for mini gel.

Caco 2 whole cell lysate (ab3950)参考文献

ab3950 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"