Cell line: Caco-2 (Human colorectal adenocarcinoma).
Growth media: Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 20% FBS.
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 5% Beta mercaptoethanol
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Whole Cell Lysate
Caco-2 cells possess many of the properties of the small intestine. As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa).
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Caco-2 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isr ecommended for mini gel.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"