Anti-CACNB2抗体[N8B/1] (ab93606)

概述

  • 产品名称
    Anti-CACNB2抗体[N8B/1]
    参阅全部 CACNB2 一抗
  • 描述
    小鼠单克隆抗体[N8B/1] to CACNB2
  • 宿主
    Mouse
  • 特异性
    No cross reactivity against CAVB1, CAVB3 and CAVB4
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    RSPKPSANSVTSPHSKE

    , corresponding to internal sequence amino acids 189 - 205 of Rat CACNB2 (NP_446303).

  • 阳性对照
    • WB: rat brain lysate
  • 常规说明

    The clone number has been updated from S8b-1 to N8B/1, both clone numbers name the same antibody clone.

     

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 克隆编号
    N8B/1
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab93606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 74 kDa).
IHC-P Use a concentration of 5 µg/ml. Antigen retrieval is not essential but may optimise staining.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.

靶标

  • 功能
    The beta subunit of voltage-dependent calcium channels contributes to the function of the calcium channel by increasing peak calcium current, shifting the voltage dependencies of activation and inactivation, modulating G protein inhibition and controlling the alpha-1 subunit membrane targeting.
  • 组织特异性
    Expressed in all tissues.
  • 疾病相关
    Defects in CACNB2 are the cause of Brugada syndrome type 4 (BRS4) [MIM:611876]. BRS4 is a heart disease characterized by the association of Brugada syndrome with shortened QT intervals. Brugada syndrome is a tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.
  • 序列相似性
    Belongs to the calcium channel beta subunit family.
    Contains 1 SH3 domain.
  • 细胞定位
    Cell membrane > sarcolemma.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AW060387 antibody
    • Ca(V) beta 2 antibody
    • CAB2 antibody
    • CACB2_HUMAN antibody
    • CACNB2 antibody
    • CACNLB2 antibody
    • Calcium channel voltage dependent subunit beta 2 antibody
    • Calcium channel voltage-dependent subunit beta 2 antibody
    • Calcium channel, voltage dependent, beta 2 subunit antibody
    • CAVB2 antibody
    • Cavbeta2 antibody
    • Cchb2 antibody
    • FLJ23743 antibody
    • Lambert Eaton Myasthenic syndrome antigen antibody
    • Lambert Eaton myasthenic syndrome antigen B antibody
    • Lambert-Eaton myasthenic syndrome antigen B antibody
    • MGC129334 antibody
    • MGC129335 antibody
    • Myasthenic (Lambert Eaton) syndrome antigen B antibody
    • Myasthenic syndrome antigen B antibody
    • MYSB antibody
    • Voltage dependent L type calcium channel subunit beta 2 antibody
    • Voltage-dependent L-type calcium channel subunit beta-2 antibody
    see all

图片

  • IHC image of ab93606 staining in Human Heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93606, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing SH-SY5Y cells stained with ab93606 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93606, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • CACNB2 immunoprecipitation from mouse skeletal muscle tissue using ab93606. 500 µg of cell lysate was incubated with undiluted primary antibody and matrix (Protein G) and incubated for 12 hours at 4°C.

    Lane 1: Mouse skeletal muscle lysate
    Lane 2: anti-CACNB2 IP

    Western blot detection: ab93606 at 1/500 dilution

    See Abreview

文献

This product has been referenced in:
  • Johnson EK  et al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013). Mouse . Read more (PubMed: 23951345) »
  • Miranda-Laferte E  et al. A Short Polybasic Segment between the Two Conserved Domains of the ß2a-Subunit Modulates the Rate of Inactivation of R-type Calcium Channel. J Biol Chem 287:32588-97 (2012). WB . Read more (PubMed: 22851179) »

See all 2 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Total protein in input
500 µg
Specification
skeletal muscle
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

I am very pleased to hear you would like to test ab93606 in IP. This code will give you $337 off your next order before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “Additional Comments” secti...

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I am sorry to read that you are still having trouble obtaining a good blot for CACNB2. Are you testing the same samples as before, rat brain homogenates? We do not have a blocking peptide for either antibody. For ab93606, you were not obtaining any sig...

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Good luck! Please let us know how it goes. If you want to submit a review, please see details at the following webpage: www.abcam.com/abreviews

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***** for ab49131. To check the status of the order please cont...

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