Anti-CACNA1S抗体[1A] (ab2862)

概述

  • 产品名称Anti-CACNA1S抗体[1A]
    参阅全部 CACNA1S 一抗
  • 描述
    小鼠单克隆抗体[1A] to CACNA1S
  • 经测试应用适用于: ICC/IF, IHC-Fr, IP, WB, IHC-P, Inhibition Assay, ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Guinea pig, Human
  • 免疫原

    Full length native protein (purified) corresponding to Rabbit CACNA1S. Purified from rabbit muscle T-tubule dihydropyridine receptor.

  • 阳性对照
    • rat skeletal muscle lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度Protein A purified
  • Primary antibody说明Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
  • 克隆单克隆
  • 克隆编号1A
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 21693436
IHC-Fr 1/200. PubMed: 21474431
IP Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 200 kDa.
IHC-P 1/20.
Inhibition Assay Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.
  • 组织特异性Skeletal muscle specific.
  • 疾病相关Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1) [MIM:170400]; also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels.
    Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
    Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1) [MIM:188580]. A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease.
  • 序列相似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.
  • 结构域Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.
  • 翻译后修饰Phosphorylation by PKA activates the calcium channel.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha-1 polypeptide antibody
    • CAC1S_HUMAN antibody
    • CACH1 antibody
    • Cach1b antibody
    • CACN1 antibody
    • CACNA1S antibody
    • CACNL1A3 antibody
    • Calcium channel antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody
    • Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody
    • Calcium channel, voltage dependent, L type, alpha 1S subunit antibody
    • Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody
    • Cav1.1 antibody
    • CCHL1A3 antibody
    • Dihydropyridine receptor antibody
    • Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody
    • fmd antibody
    • HOKPP antibody
    • HypoPP antibody
    • isoform 3 antibody
    • L type antibody
    • Malignant hyperthermia susceptibility 5 antibody
    • mdg antibody
    • MHS5 antibody
    • ROB1 antibody
    • sj antibody
    • skeletal muscle antibody
    • TTPP1 antibody
    • Voltage gated calcium channel subunit alpha Cav1.1 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1S antibody
    • Voltage-gated calcium channel subunit alpha Cav1.1 antibody
    see all

Anti-CACNA1S antibody [1A] 图像

  • Anti-CACNA1S antibody [1A] (ab2862) at 1/2000 dilution + Mouse skeletal muscle microsomes lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-mouse IgG, F(ab')2 polyclonal at 1/30000 dilution

    Performed under reducing conditions.

    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa (possible non-specific binding),36 kDa (possible non-specific binding).

    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 30 minutes at room temperature. Incubated with the primary antibody diluted in 5% skim milk in TBST for 8 hours at 4°C.

    See Abreview

  • Image from Xu X et al, PLoS One. 2010 Apr 13;5(4):e10140, Fig 8.

    Lanes 1-4 are ab2862 used to detect CACNA1S in lysate prepared from rabbit hearts. Each lane represents a separate heart.
    Frozen tissue chunks were pulverized under liquid nitrogen, and homogenized in 10 vol of buffer (0.25 M sucrose, 1 mM EDTA [pH 7.4]). This and all the following procedures took place in the presence of the protease inhibitor cocktail at 4°C or on ice. The homogenate was centrifuged at 3,500 rpm for 10 min to pellet nuclei and debris. The supernatant was centrifuged at 30,000 rpm for 1 hr to pellet the membranes. The membrane pellet was washed with a solution (Tris-HCl 20 mM [pH 7.4], EDTA 1 mM) and referred to as post-nuclear membrane fraction. The post-nuclear membrane fraction was rehomogenized in a Triton-containing lysis buffer (Tris-HCl 20 mM [pH 7.5], NaCl 0.2 M, EDTA 1 mM, Triton X-100 1%) using Dounce grinder. The mixture was centrifuged at 17,000 rpm for 1 hr, and the supernatant (Triton extract) was used for immunoblotting.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Anti-CACNA1S antibody [1A] (ab2862)参考文献

This product has been referenced in:
  • Guillaumond F  et al. Chromatin remodeling as a mechanism for circadian prolactin transcription: rhythmic NONO and SFPQ recruitment to HLTF. FASEB J : (2011). WB ; Rat . Read more (PubMed: 21507896) »
  • Kraner SD  et al. Upregulation of the CaV 1.1-ryanodine receptor complex in a rat model of critical illness myopathy. Am J Physiol Regul Integr Comp Physiol 300:R1384-91 (2011). WB, IHC-Fr ; Rat . Read more (PubMed: 21474431) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (Skeletal muscle microsomes)
Gel Running Conditions Reduced Denaturing (4–15%)
Loading amount 10 µg
Specification Skeletal muscle microsomes
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jun 15 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (C2C12 myotube)
Permeabilization Yes - 0.1% triton/PBS
Specification C2C12 myotube
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Formaldehyde
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提交于 Jun 15 2015

Thank you for your enquiry. Regarding the two antibodies, ab2862 and ab2825, I was able to find the specific antibody concentrations for you. The concentration of ab2825 (PMCA ATPase) is 7.7 mg/ml and the concentration of ab2862 (Dihydropyridine Re...

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Thank you for your enquiry. This antibody has been shown to work by Western blot; detecting a ~200 kDa protein corresponding to the DHP receptor in rat skeletal muscle extracts. You shouldn't have too much of a problem with your IP and western ...

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Thank you for your enquiry. I am sorry that ab2862 is not working for you. Since you are using the antibody in a tested application for a species that the antibody has been shown to cross-react with, this should work. Without knowing the exact p...

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Thank you for your enquiry. The concentration of ab2862 is 4.4 mg/ml.

This antibody has not been epitope mapped. The following are references in regards to IHC: Neuron, 5: 339-351, 1990. J. Cell Biol., 123(5): 1161-1174, 1993. Am. J. Physiol., 268(37): F251-F257, 1995. If you have any more questions, please co...

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