概述

  • 产品名称
    Anti-CACNA1H抗体[N55/10]
    参阅全部 CACNA1H 一抗
  • 描述
    小鼠单克隆抗体[N55/10] to CACNA1H
  • 宿主
    Mouse
  • 特异性
    No cross-reactivity against Cav1.3 and Cav3.1
  • 经测试应用
    适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Fusion protein, corresponding to amino acids 1019-1293 (II-III loop) of Human CACNA1H (accession number O95180)

  • 阳性对照
    • HEK cell lysate expressing Cav3.2
  • 常规说明

    The clone number has been updated from S55-10 to N55/10, both clone numbers name the same antibody clone.

     

性能

应用

Our Abpromise guarantee covers the use of ab84815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
WB
ICC/IF
  • 应用说明
    ICC/IF: Use at a concentration of 0.1 - 1 µg/ml. (Perox), 1 - 10 µg/ml (IF).
    IP: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 10 µg/ml. Detects a band of approximately 260 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1H gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by nickel and mibefradil. A particularity of this type of channels is an opening at quite negative potentials, and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes.
    • 组织特异性
      Expressed in kidney, liver, heart, brain. Isoform 2 seems to be testis-specific.
    • 疾病相关
      Defects in CACNA1H are a cause of susceptibility to idiopathic generalized epilepsy type 6 (IGE6) [MIM:611942]. IGE is characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain. IGE6 is a polygenic and multifactorial disease.
    • 序列相似性
      Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1H subfamily.
    • 结构域
      Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    • 翻译后修饰
      In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha1 3.2 antibody
      • Alpha13.2 antibody
      • CAC1H_HUMAN antibody
      • CACNA 1H antibody
      • CACNA1 H antibody
      • CACNA1 HB antibody
      • Cacna1h antibody
      • CACNA1HB antibody
      • Calcium channel alpha13.2 subunit antibody
      • Calcium channel voltage dependent T type alpha 1H subunit antibody
      • Calcium channel, voltage-dependent, T type, alpha 1Hb subunit antibody
      • Cav 3.2 antibody
      • Cav T.2 antibody
      • Cav3.2 antibody
      • CavT.2 antibody
      • EIG 6 antibody
      • EIG6 antibody
      • Low voltage activated calcium channel alpha 13.2 subunit antibody
      • Low voltage activated calcium channel alpha1 3.2 subunit antibody
      • Low-voltage-activated calcium channel alpha1 3.2 subunit antibody
      • MNCb 1209 antibody
      • T type Cav3.2 antibody
      • Voltage dependent t type calcium channel alpha 1H subunit antibody
      • Voltage gated calcium channel alpha subunit Cav 3.2 antibody
      • Voltage gated calcium channel alpha subunit Cav T.2 antibody
      • Voltage gated calcium channel alpha subunit Cav3.2 antibody
      • Voltage gated calcium channel alpha subunit CavT.2 antibody
      • Voltage-dependent T-type calcium channel subunit alpha-1H antibody
      • Voltage-gated calcium channel subunit alpha Cav3.2 antibody
      see all

    图片

    • ab84815 staining CACCNA1H in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

    文献

    This product has been referenced in:
    • Kürtüncü M  et al. CACNA1H antibodies associated with headache with neurological deficits and cerebrospinal fluid lymphocytosis (HaNDL). Cephalalgia : (2012). WB ; Human . Read more (PubMed: 23111027) »
    • Ohkubo T & Yamazaki J T-type voltage-activated calcium channel Cav3.1, but not Cav3.2, is involved in the inhibition of proliferation and apoptosis in MCF-7 human breast cancer cells. Int J Oncol 41:267-75 (2012). Read more (PubMed: 22469755) »

    See all 2 Publications for this product

    客户评价及客户问答

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

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    Thank you for contacting us and your interest in our products.

    Both the goat polyclonal anti-mouse IgG (ab97239 and ab98692) should be compatible with the mouse anti-CACNA1H antibody (ab84815) for the experiment you hope to perform. The antib...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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