Anti-CACNA1C抗体(ab81980)

概述

  • 产品名称Anti-CACNA1C抗体
    参阅全部 CACNA1C 一抗
  • 描述
    山羊多克隆抗体to CACNA1C
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide corresponding to Human CACNA1C aa 2201-2213 (C terminal).
    Sequence:

    C-RARGRPSEEELQD

  • 阳性对照
    • Human Heart and Skeletal Muscle lysates.

性能

应用

Our Abpromise guarantee covers the use of ab81980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).

靶标

  • 功能Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
  • 组织特异性Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
  • 疾病相关Timothy syndrome
    Brugada syndrome 3
  • 序列相似性Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
  • 结构域Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
  • 翻译后修饰Phosphorylation by PKA activates the channel.
  • 细胞定位Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha-1 polypeptide antibody
    • cardiac muscle antibody
    • isoform 1 antibody
    • L type antibody
    • CAC1C_HUMAN antibody
    • CACH 2 antibody
    • CACH2 antibody
    • CACN 2 antibody
    • CACN2 antibody
    • CACNA1C antibody
    • CACNL1A1 antibody
    • Calcium channel antibody
    • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
    • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
    • Calcium channel voltage dependent L type alpha 1C subunit antibody
    • CaV1.2 antibody
    • CCHL1A1 antibody
    • DHPR alpha 1 antibody
    • DHPR alpha 1 subunit antibody
    • LQT8 antibody
    • TS antibody
    • Voltage dependent L type calcium channel alpha 1C subunit antibody
    • Voltage dependent L type calcium channel subunit alpha 1C antibody
    • Voltage gated calcium channel alpha subunit Cav1.2 antibody
    • Voltage gated calcium channel subunit alpha Cav1.2 antibody
    • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
    • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
    see all

Anti-CACNA1C antibody 图像

  • Anti-CACNA1C antibody (ab81980) at 0.5 µg/ml + Human Heart lysate in RIPA buffer at 35 µg

    Predicted band size : 249 kDa
    Observed band size : 249 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CACNA1C antibody (ab81980)参考文献

ab81980 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply and for completing of customer survey follow up email.
I am sorry that the replacement antibody that we sent you, ab81980 did not prove to be successful for you.
Therefore I have processed your request to receive a re...

Read More

Thank you for contacting Abcam.
I have processed your request to receive ab81980 as a replacement for ab84814 and your new order number is ********.
I hope that this new antibody is successful and if there is anything else I can help you with...

Read More

Thank you for reply.
Out of either ab81980 or ab8109, I would recommend ab81980, as we have a western blot image for that one on our website. We do not have any more data for either antibody, although both are guaranteed to work in western blot on...

Read More

Thank you for your reply.
I am sorry that the protocol tips did not prove to be successful. Under our Abpromise we do guarantee this antibody for 6 months to work in western blot on human samples. Since we have not been able to resolve this issue ...

Read More

Thank you for contacting Abcam. I am sorry that you are having problems with ab84814 in western blot. I have looked over the images and protocol information that you sent and there are a few protocol changes that I would suggest that may help to reduce...

Read More

Thank you for contacting Abcam. Under our Abpromise we guarantee all of our antibodies to work as stated on the datasheet, if they do work as stated and we cannot offer any assistance to resolve the matter, then we would either replace the antibody or ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"