RabMAb

Anti-CAB39抗体[EP1680Y] (ab51132)

概述

  • 产品名称Anti-CAB39抗体[EP1680Y]
    参阅全部 CAB39 一抗
  • 描述
    兔单克隆抗体[EP1680Y] to CAB39
  • 经测试应用适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of human CAB39.

  • 阳性对照
    • HeLa cell lysate, human brain tissue, or HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab51132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/20000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).
IHC-P Use at an assay dependent concentration.

We strongly recommend that customers perform an antigen retireval step.

ICC/IF 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

靶标

Anti-CAB39 antibody [EP1680Y] 图像

  • Anti-CAB39 antibody [EP1680Y] (ab51132) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size : 40 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab51132 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51132, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Ab51132 (1:100) staining human CAB39 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
  • Immunofluorescent staining of HeLa cells using ab51132 (1:100).

Anti-CAB39 antibody [EP1680Y] (ab51132)参考文献

ab51132 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"