Anti-CA19-9抗体[SPM110] (ab15146)

概述

  • 产品名称Anti-CA19-9抗体[SPM110]
    参阅全部 CA19-9 一抗
  • 描述
    小鼠单克隆抗体[SPM110] to CA19-9
  • 经测试应用适用于: Flow Cyt, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified CA 19-9

  • 阳性对照
    • Human colon carcinoma, Human colorectal cell line C170

性能

应用

Our Abpromise guarantee covers the use of ab15146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/200.
IHC-P 1/100. Antigen retrieval is not essential but may optimise staining.

靶标

  • 别名
    • CA19.9 antibody
    • Ovarian tumor antigen antibody
    • Pancreatic tumor antigen antibody
    • Sialyl Lewis a antibody

Anti-CA19-9 antibody [SPM110] 图像

  • Immunohistochemical staining of human colon carcinoma with ab15146
  • ab15146 staining CA19-9 in the Human colorectal cell line C170 by Flow Cytometry. Cells were prepared by trypsinisation in PBS prior to incubation with the primary antibody (1 µg/ml in PBS) for 1 hour at 4°C. A FITC-conjugated Rabbit anti-mouse IgG polyclonal (1/100) was used as the secondary antibody.
    Gating Strategy: Ungated

    See Abreview

Anti-CA19-9 antibody [SPM110] (ab15146)参考文献

This product has been referenced in:
  • Sriraj P  et al. A combination of liver fluke infection and traditional northeastern Thai foods associated with cholangiocarcinoma development. Parasitol Res 115:3843-52 (2016). Read more (PubMed: 27271702) »
  • Partyka K  et al. Diverse monoclonal antibodies against the CA 19-9 antigen show variation in binding specificity with consequences for clinical interpretation. Proteomics 12:2212-20 (2012). Read more (PubMed: 22623153) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Human Serum
Gel Running Conditions Non-reduced Denaturing (4-20%)
Loading amount 15 µg
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

提交于 Jan 22 2014

I am sorry to confirm that we are not able to provide this particular antibody without BSA.

I can suggest to consider purifying the antibody using a kit to remove the BSA. For example we have the following kits available that are suitable fo...

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Thank you for contacting Abcam.

I found some information regarding the conjugation for antibodies that contain BSA through the link below:
http://www.abcam.com/index.html?pageconfig=resource&rid=13156

Our tests have found that...

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Thank you for contacting Abcam.

As requested, I have been in contact with the lab about CA 19-9 for you. The CA 19-9 is a tumor-associated antigen that was originally defined by a monoclonal antibody produced by a hybridoma prepared from mur...

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Merci de nous avoir contactés.

Malheureusement, comme nous avons plus de70.000 références anticorps à notre catalogue,pour des raisonslogistiques nous ne pouvons pas offrir de petits volumes pour des essais pr&eacu...

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Thank you for your inquiry. The immunogen was mucins from ovarian tissue and it was Protein A purified. I hope this information helps. Please contact us with any other questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colorectal carcinoma)
Specification Colorectal carcinoma
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Mar 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Human colorectal cell line C170)
Specification Human colorectal cell line C170
Preparation Cell harvesting/tissue preparation method: Typsinisation
Sample buffer: PBS
Permeabilization No
Gating Strategy Ungated
Username

Abcam user community

Verified customer

提交于 Feb 11 2011

Thank you for your enquiry. For these four antibodies, none of the epitopes have been mapped to our knowledge. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"