概述

  • 产品名称Anti-CA19-9抗体[121SLE]
    参阅全部 CA19-9 一抗
  • 描述
    小鼠单克隆抗体[121SLE] to CA19-9
  • 特异性The antibody recognizes Sialyl Lewis a containing glycolipids.
  • 经测试应用适用于: Flow Cyt, ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in human breast and human kidney tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab3982 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 22490524
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
WB Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.

靶标

  • 别名
    • CA19.9 antibody
    • Ovarian tumor antigen antibody
    • Pancreatic tumor antigen antibody
    • Sialyl Lewis a antibody

Anti-CA19-9 antibody [121SLE] 图像

  • ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).

  • All lanes : Anti-CA19-9 antibody [121SLE] (ab3982) at 5 µg/ml

    Lane 2 : Human breast tissue lysate - total protein (ab30090)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-CA19-9 antibody [121SLE] (ab3982)参考文献

This product has been referenced in:
  • Nordén R  et al. Virus-induced appearance of the selectin ligand sLeX in herpes simplex virus type 1-infected T-cells: involvement of host and viral factors. Glycobiology 23:310-21 (2013). Flow Cyt ; Human . Read more (PubMed: 23144050) »
  • Gebauer F  et al. Selectin binding is essential for peritoneal carcinomatosis in a xenograft model of human pancreatic adenocarcinoma in pfp--/rag2-- mice. Gut : (2012). Flow Cyt ; Human . Read more (PubMed: 22490524) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Melanoma cell line)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Specification Melanoma cell line
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 19 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"