Anti-CA150抗体(ab10481)
Key features and details
- Rabbit polyclonal to CA150
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CA150抗体
参阅全部 CA150 一抗 -
描述
兔多克隆抗体to CA150 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Gorilla -
免疫原
Synthetic peptide corresponding to Human CA150 aa 1-100.
Database link: 10915 -
阳性对照
- WB: HeLa and HEK293T cells. IHC-P: human breast carcinoma, mouse squamous cell carcinoma
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibodies were affinity purified using the peptide immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10481于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. -
组织特异性
Detected in brain neurons. -
序列相似性
Contains 6 FF domains.
Contains 3 WW domains. -
结构域
The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5).
The WW domains bind Pro-rich domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10915 Human
- Entrez Gene: 56070 Mouse
- Omim: 605409 Human
- SwissProt: O14776 Human
- SwissProt: Q8CGF7 Mouse
- Unigene: 723884 Human
- Unigene: 270511 Mouse
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别名
- CA 150 antibody
- Co activator of 150 kDa antibody
- Coactivator of 150 kDa antibody
see all
图片
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All lanes : Anti-CA150 antibody (ab10481) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 150 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection: DAB.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab10481 尚未被引用在任何文献中。