antigen sequence RPTRLPRRLS PFWDPATCKN LEGGAGEVVR GRDPRRLRTS RSTEILGEDL AGPSAGAAAR PAAPPPQPRE PGA, corresponding to N terminal amino acids 7-79 of Human C9orf66 (UniProt ID: Q5T8R8).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Information by UniProt
Chromosome 9 open reading frame 66 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C9orf66 antibody (ab122288)
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus but not nucleoli and focal adhesions. Recommended concentration of ab122288 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf66 antibody (ab122288)
ab122288, at a 1/175 dilution, staining C9orf66 in paraffin embedded Human breast tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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