antigen sequence: HSQFTTVRKA NQPHGAQVPR LKAALTHNPS GEGSRPCRQR CPFRVRFADE TLQDTTLRYW ERRRSVQQSV IVNQKAALPV ASERVFGSVG K, corresponding to amino acids 228-319 of Human C9orf50.
- Human cerebral cortex tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Information by UniProt
- C9orf50 antibody
- CI050_HUMAN antibody
- Uncharacterized protein C9orf50 antibody
Immunocytochemistry/ Immunofluorescence - Anti-C9orf50 antibody (ab121884)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121884 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9orf50 antibody (ab121884)
ab121884 at 1/500 dilution staining C9orf50 in Paraffin-embedded Human cerebral cortex tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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