概述

  • 产品名称Anti-C9orf172抗体
    参阅全部 C9orf172 一抗
  • 描述
    兔多克隆抗体to C9orf172
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rhesus monkey, Gorilla
  • 免疫原

    amino acids 307-400 of Human C9orf172.

  • 阳性对照
    • Hman bone marrow tissue; RT4, U251 MG cell lysates, Human liver tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20ºC.
  • 存储溶液pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab121654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

Anti-C9orf172 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoplasm and focal adhesions. Recommended concentration of ab121654 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
    Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
  • All lanes : Anti-C9orf172 antibody (ab121654) at 1/500 dilution

    Lane 1 : RT4 cell lysates
    Lane 2 : U251 MG cell lysates
    Lane 3 : Human Plasma
    Lane 4 : Liver tissue lysate
    Lane 5 : Tonsil tissue lysate
  • ab121654, at 1/150 dilution, staining C9orf172 in Human bone marrow tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

Anti-C9orf172 antibody (ab121654)参考文献

ab121654 has not yet been referenced specifically in any publications.

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